Abstract: Animals can make adaptive adjustment of selectivity as a function of their own reproductive value that may cause variation in mate choice over the course of reproduction. Procambarus. clarkii are a species rich group of large decapods crustaceans showing a promiscuous breeding system, with both sexes copulating repeatedly. Previously, plenty of papers discussed the effects of body size and hierarchical status on mutual mate choice in P clarkii, while few of them paid attention to mating status as a criterion of sexual selection. Because of the long time retention between mating and oviposition, their mating status will change accordingly. On the basis of former investigation of mate choice in virgin males, in this study, to examine whether choosers’ mate choice are influenced by their own mating history, we manipulated the reproductive condition of crayfish and allowed mated males crayfish to make choice among opposite sex with different mating status. By means of a Y maze, the chooser made sexual selection between two targets with contrasting mating status. P. clarkii males with mating experience discriminated between partners of the same body size but with different mating status in a Y maze. Mated males spent significant more time visiting their primary partners rather than virgin targets in the total and mean durations of visits (P<0.05), but not in the number of visits (Two-tailed test P=0.086, but one-tailed test P=0.043). When there was no existence of primary partners, males randomly made mate choice between virginity and nonvirgin females. When both potential mates had mating experience, males chose primary partners and nonvirgin females indiscriminately. The results showed P. clarkii males are partly able to recognize mating status of potential mates, and further indicated that male crayfish probably guarantee their own paternity by mate guarding based on their borrowing behavior. However, males may regulate their loyalty to primary partners whether they exist or not. In conclusion, males are able to dynamically adjust mate choice based on their own mating history and costs of mate-getting