Abstract: The field observations for turbulence characteristics in the urban roughness sublayer were conducted on the meteorological tower in Nanjing Municipal Party School. The analysis of power spectra of wind speed at the two levels shows that: (1) In near neutral condition s, the two levels’ turbulence kinetic energy density is larger than that near homogeneous grass land , and the value at the lower level is larger than that at the higher level. The t urbulence at the lower level is more anisotropic than that at the higher level. The production of the turbulence is stronger over the urban canopy than that over the homogeneous grass land , and the value at the lower level is also larger than that at the higher level. About 20% of the spectra has a spectra gap. The mean scale of the secondary s pectra p eaks is similar with the scale of the roughness elements, which means that the individual element has prominent effect on turbulence in urban roughness sublayer. (2) In near neutral conditions, turbulence kinetic energy dissipation rate at the two levels are higher than the result over the h omogeneous grass land. As to the two levels ’ results, t urbulence kinetic energy dissipation rate is higher at low level. In unstable conditions, the t urbulence kinetic energy dissipation rate grows faster with increasing instability than the result observed over the h omogeneous grass land. In stable condition s, the growing rates of t urbulence kinetic energy dissipation rate with increasing stability over the two kind surfaces are similar