腐植酸对芘的生物毒性效应的影响*

南京大学学报(自然科学版) ›› 2012, Vol. 48 ›› Issue (6): 774–779.

腐植酸对芘的生物毒性效应的影响*

蒋丽娟¹，尹颖¹**，尹大强²,郭红岩¹，杨柳燕¹，王晓蓉¹

出版日期:2015-07-29 发布日期:2015-07-29
作者简介: (1.污染控制与资源化研究国家重点实验室，南京大学环境学院，南京，210046;
2.长江水环境教育部重点实验室，同济大学环境科学与工程学院，上海，200092)
基金资助:
黄河流域公益性项日(201001010)，国家自然科学基金(20907020)

The influence of humic acid on pyrene biotoxicity in
liver of Carassius auratus

Jiang Li-Juan¹，Yin Ying¹，Yin Da-Qiang²，Guo Hong-Yan¹，Yang Liu-Yan¹，Wand Xiao-Rong¹

Online:2015-07-29 Published:2015-07-29
About author: (1 .State Key Laboratory of Pollution Control and Resources Reuse，Academy of Environment,
Nanjing University,Nanjing,210046,China;
2. Laboratory of Yangtze River Water Environment，Ministry of Education, School of Environmental Science
and Engineering,Tongji University,Shanghai,200092,China)

摘要/Abstract

摘要： 本文旨在研究水体中腐植酸(humic acid, HA)对芘胁迫下鲫鱼肝脏中富集花，活性氧的诱导及氧化损伤的影响.通过静态模拟实验，在水体中不同浓度(0,0. 05,0. 1,0. 5, 1. 0和2. 5 mg/I.)的HA与芘(0.05 mg/I.)共同暴露10天后，取鲫鱼肝脏，分析其中芘的富集量、活性氧自由基的诱导信号、脂质过氧化产物丙二醛(MDA)含量以及抗氧化系统发生的变化.结果发现，随着HA浓度的增加，花在鲫鱼月+脏中的富集降低;0. 06mg/L和0. 1 mg/L的HA显著诱导鲫鱼肝脏产生活性氧自由基，但随着HA浓
度升高，这种诱导效应降低;0. 6 mg/Z,和1. 0 mg/L H A可以使肝脏中MDA含量显著降低;三种抗氧化系统酶超氧化物歧化酶，过氧化氢酶，谷肤甘肤一分转移酶活性及谷肤甘肤含量因HA的加入均显示先升高后降低的趋势.本文认为，HA降低芘在螂角肝脏中的富集，并改变芘对鲫鱼的生物效应，减轻芘对鲫鱼产生的氧化损伤.

Abstract: In the current study,whether the different concentrations of humic acid(HA)may influence bioaccumula- tion and oxidative stress of pyrene in liver of Curussius uurutus was investigated.The fish(C. uurutus)was exposed to HA(0,0.05，0. 1 , 0. 5，1.0 and 2. 5 mg/L)and pyrene(0. 05 mg/L)mixturcs for 10 days.Then the pyrene bioaccumu- lation as well as their effects on the reactive oxygen species，Malondialdchyde(MDA)contcnt and antioxidativc sys- tem in the liver of C.uurutus were analyzed. With the HA concentration increase, bioaccumulation of pyrene in liver of C.uurutus reduced. In the mcantime,0. 05 mg/Land 0. 1 mg/L HA could induce the production of reactive oxygen species in the liver of C.uurutus，0. 5 mg/L and 1. 0 mg/L HA could decrease the MDA contents.The superoxide dismutasc, catalasc，glutathionrs-transferase activities and glutathione contents decreased after an initial increase，under HA concentration increase. It was pyrene stress which caused the oxidative damage of the liver and brain, which could be further induced by HA. Overall，our present work illustrated that the presence of HA could reduce pyrene concentration in fish liver and change the pyrene’s biological effects，which should not be disregarded when
considering the ecological safety of pyrene in the aquatic system.

蒋丽娟¹，尹颖¹**，尹大强²,郭红岩¹，杨柳燕¹，王晓蓉¹. 腐植酸对芘的生物毒性效应的影响*[J]. 南京大学学报(自然科学版), 2012, 48(6): 774–779.

Jiang Li-Juan¹，Yin Ying¹，Yin Da-Qiang²，Guo Hong-Yan¹，Yang Liu-Yan¹，Wand Xiao-Rong¹
. The influence of humic acid on pyrene biotoxicity in
liver of Carassius auratus
[J]. Journal of Nanjing University(Natural Sciences), 2012, 48(6): 774–779.

参考文献

[1]Mai B X,Fu J M,Sheng G Y,et al. Chlorinated and polycyclic aromatic hydrocarbons in rivcrinc and es- tuarine sediments from Pearl River De1ta,China. En-
vironmental Pollution,2002，117:457一474.
[2]Wu Y，Zhang J，Zhu Z J. Polycyclic aromatic by drocarbons in the sediments of the Yalujiang Es tuary, North China.
Marine Pollution Bulletin2003.46.619一625.
[3]Chen B L，Xuan X D,Zhu L Z,etal. Distributions of polycyclic aromatic hydrocarbons in surface waters, sediments and soils of Hangzhou City,
China. Water Research,2004，38:3558~3568.
[4]Levengood J M,Schaeffer D J. Polycyclic aromatic hy- drocarbons in fish and crayfish from the Calumet re- gion of southwestern Lake Michigan. Ecotoxicology,
2011,20:1411一1421.
[5]Strickland P, Kang D. Urinary 1-hydroxypyrene and other PAH metabolites as biomarkers of exposure to environmental PAH in air particulate matter. Toxico-
logical Letters, 1999，108:191一199.
[6]Yin Y，Sun Y Y，Guo H Y，et al. Bio-toxic effect of pyrene on Vallisneria spiralis. Chinese Journal of Applied Ecology, 2007，7(18):1528一1533.
(尹颖，孙媛媛，郭红岩等.芘对苦草的生物毒性效应.应用生态学报，2007 , 7 ( 18 ) : 1528 - 1533).
[7]Sun Y Y, Yin Y, Zhang J F, et al. Hydroxyl radical generation and oxidative stress in Carassius auratus liver, exposed to pyrene. Ecotoxicology and Environ-
mental Safetyh,2008,71:446~453.
[8]Yin Y, Wang X R, Sun Y Y, et al. Bioaccumulation and oxidative stress in submerged macrophyte Cera- tophyllum demersum L, upon exposure to pyrene.
Environmental Toxicology , 2008 , 23:328一336.
[9]Chiou C T，Malcolm R L. Water solubility en- hancements of some organic pollutants and pesti- cides by dissolved humic and fulvic acids. Envi-
ronmental Science and Technology,1986，20:502 ~507.
[10]Oris J T，Hall A T，Tylka J D. Humic acids re- duce the photo-induced toxicity of anthracene to fish and Daphnia. Environmental Toxicology and
Chemistry, 1990，9:575一583.
[11]Lee S K，Freitag D, Steinberg C，et al. Effects of dissolved humic materials on acute toxicity of some organic chemicals to aquatic organisms. Wa-ter Research，1993,27:199~204.
[12]Zhou Z L,Sun H W,Zhang W. Dcsorption of pol- ycyclic aromatic hydrocarbons from aged and un- aged charcoals with and without modification of
humic acids. Environmental Pollution, 2010，158: 1916一1921.
[13]Shi H H, Wang X R, Luo Y. Electron paramagnetic resonance evidence of hydroxyl radical generation and oxidative damage induced by tetrabromobisphenol A
in Caraeeius auratus. Aquatic Toxicology，2005，74: 365一371.
[14]Hissin P J，Hilf R,A fluorometric method for deter- urination of oxidized and reduced glutathione in tis- sues. Analytical Biochemistry, l 976 , 74; 214一226.
[15]Xu J P, Yuan X F, Lang P Z. The determination of cnzymic activity and its inhibition on catalasc by ul- traviolet spectrophotometry. Environmental Chemis-
try,1997,16(1);73-76.(徐镜波，袁晓)·L，郎佩珍.过氧化氢酶活性及活性抑制的紫外分光光度法测定.环境化学，1997,16(1);73-76).
[16]Marklund S,Marklund G.Involvement of the su- peroxide anion radical in the autooxidation of py- rogallol and a convenient assay for superoxide
dismutase. European Journal Biochemistry, 1974，47:469一474.
[17]Habig W H , Pabst M J , Jakoby W B. Glutathione S-transferases;the first enzymatic step in mercap- turic acid formation. Journal of Biological Chemis-
try,1973,249:7130一7139.
[18]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utili- zing the principle of protcirrdyc binding. Analytical
Biochemistry, 1976，72;248一254.
[19]Ohkawa H，Ohisi N, Yagi K. Assay for lipid per- oxides in animal tissues by thiobarbituric acid re action. Analytical Biochemistry, 1979，95:351 -358.
[20]Zhu Y, Dai J Y. Research progress in the structure characterization of humus and its environmental meaning. Chinese Journal of Soil Science, 2006，37
(6):1224-1230.(朱燕，代静玉.腐殖物质对有机污染物的吸附行为及环境学意义.土壤通报，2006，37 (6):1224一1230).
[21]Xu Y，Wu W Z,Zhang Y Y. Effect of aquatic hu- mic acids on bioavailability and acute toxicity of meothrin. Acta Hydrobiologica Sinica, 1996，20
(2) ; 160-163.(徐盈，吴文忠，张甫元.水体腐殖酸对甲氰菊醋的生物可利用性与急性毒性的影响.水生生物学报，1996,20(2);160-163).
[22]Cao L,Sun Y Y, Wang X R,et al. Effects of humic acid on bioaccumulation and oxidative stress induced by tetrabromobisphenol A in coontail Ceratophyllum
demersum. L. Journal of Agro-Environment Science, 2009,28(3):476-480.曹璐，孙媛媛，王晓蓉等.腐植酸对四嗅双酚A在金角藻体内的富集及
氧化胁迫的影响.农业环境科学学报，2009,28 (3) :476一480) .
[23]Fabian N J，Albright L B,Gerlach G,etal. Humic acid interferes with species recognition in Ze- brafish(Danio rerio).Journal of Chemical Ecolo-
gy,2007，33:2090一2096.
[24]Honkanen JO，Wiegand C,Kukkonen J V K. Hu-mic substances modify accumulation but not bio- trans-formation of pyrene in salmon yolk-sac fry
Aquatic Toxicology, 2008，86:29一248.
[25]Wang X R，Luo Y,Shi H H,et al. Application of molecular biomarkers in early diagnosis and eco- logical risk assessment for water and soil. Envi- ronmental Chemistry, 2006，25(3):320一325.(王晓蓉，罗义，施华宏等.分子生物标志物在污染环境早期诊断和生态风险评价中的应用.环境化学，2006,25(3):320一325).
[26]Zhou C F,Qin P,Xie M. Free radical responses m embryonic calli of Spartina patens under N+ beam irradiation. Journal of Nanjing University(Natu-
ral Sciences),2003,39(5):522-530.(周长芳，钦佩，谢民.N^十离子束注入对狐朱草肛性愈伤组织自由基代谢的影响.南京大学学报(自然科学)，2003，39(5)522一530)

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