利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率*

南京大学学报(自然科学版) ›› 2011, Vol. 47 ›› Issue (2): 155–163.

利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率*

赵瑾瑜 1 , 渠敏 1 , 李家璜 2 , 张?铮 3 , 张淑仪 1 ** , 水修基 1 , 杨跃涛 1 , 华子春 2

出版日期:2015-04-09 发布日期:2015-04-09
作者简介: ( 1. 南京大学声学研究所, 近代声学实验室, 南京, 210093;
2. 南京大学生命科学院, 医药生物技术实验室, 南京, 210093;
3. 南京市第一医院, 南京大学医学院附属医院, 南京, 210006)
基金资助:
国家自然科学基金( 11074125)

Quantum yields in photolyses of mammalian carboxy -hemoglobin studied by pulsed laser pump -probe technique

Zhao Jin -Yu 1 , Qu Min 1 , Li Jia -H uang 2 , Zhang Zheng 3 , Zhang Shu -Yi 1* * , Shu X iu -J i 1 , Yang Yue-T ao 1 , Hua Zi-Chun

Online:2015-04-09 Published:2015-04-09
About author: ( 1. Laboratory of Modern Acoustics, Institute of Acoustics, 2. Laboratory of Pharmaceutical Biotechnology,
Institute of Life Science, 3. Nanjing First Hospital, Attached to School of Medicine,
Nanjing University, Nanjing, 210006, China)

摘要/Abstract

摘要： 本文利用脉冲光解法测量了哺乳动物碳氧血红蛋白的光解反应的量子产率. 脉冲光解法是利用一束波长为 532 nm、脉冲宽度为 8 ns、重复频率为 10 Hz 的脉冲激光照射碳氧血红蛋白溶液, 使其发
生光解反应. 考虑到碳氧血红蛋白溶液和其光解产物脱氧血红蛋白的光吸收系数不同, 可利用另一束波长为 432nm 的连续激光检测溶液在光解前、后的透射率的变化, 以测定溶液的光解量子产率. 利用此实
验系统, 对人、猪、牛、马和兔等 5 种哺乳动物的碳氧血红蛋白的光解量子产率进行了测量和研究, 结果表明, 不同物种的光解量子产率各不相同, 其中猪、牛、马的碳氧血红蛋白的光解量子产率很接近, 兔的
碳氧血红蛋白的光解量子产率与其他物种的差异最大. 最后, 对测量的结果从血红蛋白的氨基酸序列、氢键和盐桥排布、四级结构等方面进行了分析与讨论.

Abstract: Hemoglobin ( Hb) as the allosteric protein, in photo -dissociations of liganded Hb has been studied widely. T he mechanisms describing the cooperative binding of CO and other ligands to hemoglobins has been the
subject of extensive studies as an important fundamental problem for a long time. In this paper, the quantum yields in photolyses of carboxy -hemoglobins ( HbCO) of mammals, such as human,
pig, bovine, horse and rabbit, are investigated by the optical pump -probe technique, in which the quantum yield is defined as the molecular number of photoproduct species divided by the absorbed photon number. In the optical
pump -probe technique, the HbCO of the mammals are irradiated by a pulsed pumping laser beam with the wavelength 532 nm, pulse width 8 ns and the repetition frequency 10 Hz, then the HbCO is photo -dissociated.
Meanwhile, another continuous optical beam with the wavelength 432 nm is used as a probe beam to detect the absorbance change induced by the photo -dissociation before and after the laser pulse illuminating HbCO. In the
experiments, a solution with the recognized quantum yield 0 is taken as a standard solution, and adjust the optical absorption ( at the wavelength 532 nm) of the measured solutions to be the same data as that of the standard
solution, then the quantum yield of the measured solution can be obtained by comparing the variations of the optical absorption ( at 432 nm) induced by the photolyses of the solutions with those of the standard solution
measured at the same experimental conditions. The measured results show that the quantum yields o the photolyses of the HbCO are different for varies
mammals, but for three of the mammals, i. e. , pig, bovine and horse, the photolysis quantum yields of their HbCO are very close. However, for the HbCO of rabbit, the quantum yield is much smaller than the others. Finally, the
results are analyzed and discussed. To explain the differences among them, some analyses and discussions on the differences of the amino acid sequences of the five Hb, and the tetramer structures, as well as the salt bridges and
hydrogen bonds between subunits of HbCO among the five mammals are presented.

赵瑾瑜 1 , 渠敏 1 , 李家璜 2 , 张?铮 3 , 张淑仪 1 ** , 水修基 1 , 杨跃涛 1 , 华子春 2 . 利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率*

[J]. 南京大学学报(自然科学版), 2011, 47(2): 155–163.

Zhao Jin -Yu 1 , Qu Min 1 , Li Jia -H uang 2 , Zhang Zheng 3 , Zhang Shu -Yi 1* * , Shu X iu -J i 1 , Yang Yue-T ao 1 , Hua Zi-Chun . Quantum yields in photolyses of mammalian carboxy -hemoglobin studied by pulsed laser pump -probe technique

[J]. Journal of Nanjing University(Natural Sciences), 2011, 47(2): 155–163.

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