Pokemon 在人贲门癌中的表达及其意义

南京大学学报(自然科学版) ›› 2010, Vol. 46 ›› Issue (1): 108–113.

• • 上一篇

Pokemon 在人贲门癌中的表达及其意义

曹国春 1,2 , 王? 会 1 , 黄亚玲 1 , 王庆玲 1 , 侯亚义 1,3

出版日期:2015-03-27 发布日期:2015-03-27
作者简介:( 1. 南京大学医学院免疫与生殖实验室, 医药生物技术国家重点实验室, 南京, 210093;
2. 江苏省肿瘤医院, 南京, 210009;
3. 江苏省医学分子技术重点实验室, 南京, 210093)
基金资助:
Key Science Research Foundation of Jiangsu Province ( BS2007010) , the Foundation for Key Program of M inistry of Education, China ( 02111) .

Expression of Pokemon in human esophagogastric junctional cancer *

Cao Guo -Chun 1, 2 , Wang Hui 1 , H uang Ya -Ling 1 , Wang Qing-Ling 1 , Hou Ya -Yi 1, 3 **

Online:2015-03-27 Published:2015-03-27
About author: (1. Imunology and Reproduction Biology Laboratory of Medical School, State Key Laboratory of
Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China;
2. Jiangsu Cancer Hospital, Nanjing, 210009, China;
3. Jiangsu Key Laboratory of Molecular Medicine, Nanjing, 210093, China)

摘要/Abstract

摘要： ? Pokemon 基因是一种转录抑制因子, 它在癌症发生发展过程中起重要作用. 目前已发现 Pokemon 在人淋巴瘤、乳胶癌、肺癌、结肠癌、前列癌以及膀胱癌中高表达. 但是在贲门癌中表达水平未见报道. 本研究采用逆转
录多聚酶链反应( RT -PCR)和实时定量多聚酶链反应(rea- l time PCR) 方法检测了 56 例贲门癌( EGJ) 和 10 例正常人贲门组织标本的 Pokemon 基因 mRNA 表达水平. 结果显示所有组织标本均表达 Pokemon. 但是肿瘤组织及其配
对的远离肿瘤的瘤旁组织 Pokemon 基因的 mRNA 表达水平显著高于正常人的贲门组织( P = 0. 000 2, P=0. 006 9) . 结果显示 Pokemon 基因可能对贲门癌的发生发展及致癌性转化有重要作用.

Abstract: ? Human Pokemon, a transcriptional factor, lays a key role in cancer pathogenesis. It was found that Pokemon expression was at a high level in a subset of human lymphomas, breast, lung,
colon, prostate and bladder carcinomas. However, no information available about Pokemon expression in human esophagogastric junctional (EGJ) cancers. In this study, we detected the expression of Pokemon
gene in 56 surgically resected specimens of esophagogastric junctional ( EGJ) cancers as well as 10 tissues of nonneoplastic disease using the reverse transcriptase -polymerase chain reaction ( RT -PCR) and rea- l
time PCR. The results showed that all of the samples expressed Pokemon. The median relative mRNA expression of the Pokemon genes was significantly higher in tumor tissues as compared to normal tissue
samples (P= 0. 000 2) and in corresponding non -cancerous tissues as compared to normal tissue samples (P= 0. 006 9). T hese results suggest that the Pokemon expression levels are necessary for oncogenic
transformation and may be associated with tumorigenesis.

曹国春 1,2 , 王? 会 1 , 黄亚玲 1 , 王庆玲 1 , 侯亚义 1,3
. Pokemon 在人贲门癌中的表达及其意义
[J]. 南京大学学报(自然科学版), 2010, 46(1): 108–113.

Cao Guo -Chun 1, 2 , Wang Hui 1 , H uang Ya -Ling 1 , Wang Qing-Ling 1 , Hou Ya -Yi 1, 3 **
. Expression of Pokemon in human esophagogastric junctional cancer *
[J]. Journal of Nanjing University(Natural Sciences), 2010, 46(1): 108–113.

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Pokemon 在人贲门癌中的表达及其意义

Expression of Pokemon in human esophagogastric junctional cancer *

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