南京大学学报(自然科学版) ›› 2010, Vol. 46 ›› Issue (1): 100–107.
汤亚红 1 , 徐力致 1 , 汤新慧 2 , 戴安怡 1 , 赵晓宁 1 , 严丽芳 3 , 高 静 3
Tang Ya 2 Hong 1 , X u L i 2 Zhi 1 , Tang Xin 2 Hui 2 , Dai A n 2 Yi 1 , Zhao Xiao 2 N ing 1 , Yan L i 2 Fang 3 , Gao J ing 3
摘要： 探讨中华补血草水提物 (LSE) 对抗 N -乙酰对氨基酚(N-acetyl - p - aminophenol ,APAP) 引起的肝细胞损伤的作用及其可能的线粒体机制 . 通过分组实验 , 测定血清中谷丙、 谷草转氨酶 (sAL T ,sAST) 的活性和谷胱甘肽
(GSH) 的含量 , 同时制备和观察肝组织切片 . 其后进一步探讨线粒体在这一过程中所发生的变化 , 通过测定线粒体的肿胀、 线粒体膜电位 (MMP) 的变化以及电压依赖性离子通道 (VDAC) 在转录水平的变化等 , 探讨其相应的线粒
体作用机制 . 结果显示 :100 、 200 、 400 mg/ kg 的 LSE 可以剂量依赖性地对抗 APAP 引起的肝细胞损伤 , 而这种护肝活性可能与 LSE 对肝细胞线粒体的保护作用有关 . 同时还发现 , 线粒体的一个重要的外膜蛋白 — — — VDAC 的表达
可能与之有着密切的关系 .
| [ 1 ] Lin L C , Chou C J. Flavonoids and phenolics from L imonium sinense . Phytotherapy Re -search , 2000 , 66 (4) : 382. [ 2 ] Chaung S S , Lin C C , Lin J. The hepatoprotec -tive effects of Limonium sinense against carbon tetrachloride and beta -D -galactosamine intoxica -tion in rats. Phytotherapy Research , 2003 , 17(7) : 784 ～ 791. [ 3 ] Tang X H , Gao J , Chen J , et al . Expression of AC regulated by extracts of Limonium sinense Ktze root against CCl4 - induced liverdamage. International Journal of Molecular Sci -ences , 2007 , 8(3) : 204 ～ 213. [ 4 ] Tang X H , Gao J. Mitochondrial modulation is involved in the hepatoprotection of Limonium sinense extract against liver damage in mice.Journal of Ethnopharmacology , 2008 , 120 (3) :427 ～ 431. [ 5 ] Aprille J R , Hom J A , Rulfs J. Liver and skel -etal muscle mitochondrial function following burn injury. Journal of Trauma , 1977 , 17 (4) :279 ～ 287. [ 6 ] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein - dye binding. Analytical Biochemistry , 1976 , 72 (1 22) : 248 ～ 254. [ 7 ] Emaus R K, Grunwald R , Lemasters J J. Rho -damine 123 as a probe of transmembrane poten -tial in isolated rat - liver mitochondria : Spectral and metabolic properties. Biochimica et Bio -physica Acta - Bioenergetics , 1986 , 850 ( 3 ) :436 ～ 448. [ 8 ] Wu E Y, Smith M T , Bellomo G. Relationships between the mitochondrial transmembrane po -tential , ATP concentration ,and cytotoxicity in isolated rat hepatocytes. Architecture Biochem -istry Biophysiology , 1990 , 282(2) : 358 ～ 362. [ 9 ] Bleibel W , Kim S , D ’ Silva K, et al . Drug - in -duced liver injury: Review article. Digestive Diseases and Sciences , 2007 , 52 ( 10 ) :2463 ～ 2471. [10] Gunawan B K, Kaplowitz N. Mechanisms of drug -induced liver disease. Clinics in Liver Dis ease , 2007 , 11(3) : 459 ～ 475. [11] Armstrong J S. Mitochondrial medicine : Phar -macological targeting of mitochondria in dis -ease. British Journal of Pharmacology , 2007 ,151(8) : 1154 ～ 1165. [12] Kuvandik G, Duru M. Effects of erdosteine on acetaminophen -induced hepatotoxicity in rats.Toxicologic Pathology ,2008 ,36(5) : 714 ～ 719. [13] Jaeschke H , Cover C , Bajt M L. Role of caspases in acetaminophen - induced liver injury.Life Sciences , 2006 , 78(15) : 1670 ～ 1676. [14] Jaeschke H , Knight T R , Bajt M L. The role of oxidant stress and reactive nitrogen species in acetaminophen hepatotoxicity. Toxicology Let -ters , 2003 , 144(3) : 279 ～ 288. [15] Masubuchi Y, Suda C , Horie T. Involvement of mitochondrial permeability transition in acet -aminophen -induced liver injury in mice. Journal of Hepatology , 2005 , 42(1) : 110 ～ 116. [16] Kon K, Kim J S , Jaeschke H , et al . Mitochon -drial permeability transition in acetaminophen -induced necrosis and apoptosis of cultured mouse hepatocytes. Hepatology , 2004 , 40(5) :1170 ～ 1179. [17] Premkumar A , Simantov R. Mitochondrial voltage - dependent anion channel is involved in dopamine - induced apoptosis. Journal of Neuro -chemistry , 2002 , 82(2) : 345 ～ 352. |
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