南京大学学报(自然科学版) ›› 2024, Vol. 60 ›› Issue (1): 168–180.doi: 10.13232/j.cnki.jnju.2024.01.017

• • 上一篇    

不同浸种及催芽处理对明日叶种子萌发的影响

周泽妍1, 宗树斌3, 谭海霞2, 田兴军1()   

  1. 1.南京大学生命科学学院,南京,210023
    2.河北省农业生态安全重点实验室, 河北环境工程学院,秦皇岛,066102
    3.江苏农林职业技术学院, 镇江,212400
  • 收稿日期:2023-10-30 出版日期:2024-01-30 发布日期:2024-01-29
  • 通讯作者: 田兴军 E-mail:tianxj@nju.edu.cn
  • 基金资助:
    江苏省林业科技创新与推广项目(LYKJ[2021]16);国家自然科学基金(31870598)

Effects of different soaking and germination accelerating treatments on seed germination of Angelica keiskei

Zeyan Zhou1, Shubin Zong3, Haixia Tan2, Xingjun Tian1()   

  1. 1.School of Life Science, Nanjing University, Nanjing, 210023, China
    2.Hebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao,066102,China
    3.Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang, 212400, China
  • Received:2023-10-30 Online:2024-01-30 Published:2024-01-29
  • Contact: Xingjun Tian E-mail:tianxj@nju.edu.cn

摘要:

明日叶(Angelica keiskei)是一种经济价值极高、开发前景广阔的食药两用蔬菜.然而由于土壤及温湿度条件等因素限制,明日叶种子出苗率低,制约明日叶的栽培生产.以山东新产的绿茎明日叶种子为材料,通过不同水温浸种及不同浓度KNO3、植物激素、强氧化剂的外源添加,研究了提高种子的萌发处理方法.结果表明,温水(55 ℃)浸种、KNO3溶液、植物激素、强氧化剂处理均可不同程度地促进种子萌发.与清水浸种相比,在KNO3溶液处理组中,750 mg·L-1处理的发芽率和发芽势分别为83.82%和59.56% (p<0.05),为组内最高;在植物激素处理组中,100 mg·L-1 GA3溶液处理发芽率最高,达到81.19% (p<0.05);在强氧化剂处理组中,500 mg·L-1 KMnO4溶液处理的发芽率和发芽势分别为84.92%和46.03% (p<0.05),为组内最高.在这三种外源添加处理条件下,750 mg·L-1KNO3溶液处理的幼苗下胚轴长1.08 cm,根长2.09 cm;100 mg·L-1 GA3溶液处理的幼苗下胚轴长2.41 cm,根长2.01 cm;500 mg·L-1 KMnO4溶液处理的幼苗下胚轴长度1.21 cm,根长1.63 cm.综合比较发现,100 mg·L-1 GA3溶液处理条件下明日叶种子发芽率高,且植株幼苗的根长及下胚轴长度也最好.本研究结果可为明日叶种植及同类种子繁育提供借鉴.

关键词: 明日叶, 温水浸种, 植物激素, 强氧化剂, 种子萌发

Abstract:

Angelica keiskei is an edible and medicinal vegetable with high economic value and broad development prospects. However,due to the limitation of soil properties,temperature and humidity conditions,the germination rate of Angelica keiskei seeds is generally low,which restricts the cultivation and production of Angelica keiskei. In this study,the seeds of Angelica keiskei,which was newly harvested in Shandong Province,were used as materials to study how to improve the germination of seeds by soaking seeds at various temperature water and exogenous addition of different concentrations of KNO3,plant hormones and strong oxidants. The results show that soaking seeds in warm water (55 ℃),KNO3 solution,plant hormones and strong oxidant treatment could promote seed germination to varying degrees. Compared with water soaking,in the KNO3 solution treatment group,the germination rate and germination potential ability of 750 mg·L-1 treatment were 83.82% and 59.56% (p<0.05),respectively,which were the best in the group. In the plant hormone treatment group,the germination rate of 100 mg·L-1 GA3 solution treatment was the highest,reaching 81.19% (p<0.05). In the strong oxidant treatment group,the germination rate and germination potential of 500 mg·L-1 KMnO4 solution were 84.92 % and 46.03 %,respectively (p< 0.05 ),which was the highest in the group. The hypocotyl length of seedlings treated with 750 mg·L-1 KNO3 solution was 1.08 cm,the root length was 2.09 cm,the hypocotyl length of seedlings treated with 100 mg·L-1 GA3 solution was 2.41 cm,the root length was 2.01 cm,and the hypocotyl length of seedlings treated with 500 mg·L-1 KMnO4 solution was 1.21 cm,the root length was 1.63 cm. Comprehensive comparison above results,we suggested that the treatment of 100 mg·L-1 GA3 solution was the best treatment due to high seed germination rate,the largest root length and hypocotyl length of the seedlings. The results of this study can provide reference for Angelica keiskei planting and breeding of similar seeds.

Key words: Angelica keiskei, lukewarm?water?soaked, plant hormone, strong oxidizer, seed germination

中图分类号: 

  • Q945

表1

明日叶种子的不同温水浸种处理"

浸种温度(℃)催芽温度(℃)催芽光照条件(光照强度单位:lx)
25 (Control)25 (Control)0
4515,22,300
5515,22,300
6515,22,300
55150
153000
15催芽处理后直接播种进土壤

表2

明日叶种子的KNO3溶液及五种植物激素浸种处理"

处理浓度(mg·L-1
KNO3溶液0,10,50,100,250,500,750,1000
GA3溶液0,50,100,200,300,400
NAA溶液0,50,100,200,300
SA溶液0,100,200,300,400
IAA溶液0,20,50,100,200
6⁃BA溶液0,5,10,20,30,50,100,200,300

表3

明日叶种子的三种强氧化剂催芽处理"

处理浓度(mg·L-1浸种时间
Control012 h
KMnO4溶液100,250,500,1000,300020 min
H2O2溶液

3×103,1×104,3×104,5×104,

1×105,3×105

20 min
NaClO溶液100,1000,1000020 min

图1

不同浸种温度及发芽温度对明日叶种子发芽的影响"

图2

不同浓度KNO3溶液处理对明日叶种子发芽的影响"

图3

不同浓度KNO3溶液处理对明日叶幼苗生长的影响"

图4

五种植物激素及其不同浓度梯度处理对明日叶种子发芽的影响"

图5

不同植物激素处理对明日叶幼苗生长的影响"

图6

不同强氧化剂及其不同浓度处理对明日叶种子发芽的影响"

图7

不同强氧化剂处理对明日叶幼苗生长的影响"

1 Kawabata K, Sawada K, Ikeda K,et al. Prenylated chalcones 4?hydroxyderricin and xanthoangelol stimulate glucose uptake in skeletal muscle cells by inducing GLUT4 translocation. Molecular Nutrition & Food Research201155(3):467-475.
2 李佳,张智俊,周长芳,等. 明日叶的组织培养与快速繁殖. 植物生理学通讯200642(6):1142.
Li J, Zhang Z J, Zhou C F,et al. Tissue culture and rapid propagation of Angelica keiskei Koidz. Plant Physiology Communications200642(6):1142.
3 Zhang K L, Zhang Y, Walck J L,et al. Non?deep simple morphophysiological dormancy in seeds of Angelica keiskei (Apiaceae). Scientia Horticulturae2019,255:202-208.
4 Enoki T, Ohnogi H, Nagamine K,et al. Antidiabetic activities of chalcones isolated from a Japanese herb,Angelica keiskei . Journal of Agricultural and Food Chemistry200755(15):6013-6017.
5 Mottin M, Caesar L K, Brodsky D,et al. Chalcones from Angelica keiskei (ashitaba) inhibit key Zika virus replication proteins. Bioorganic Chemistry2022,120:105649.
6 Rawat A, Reddy A V B. Recent advances on anticancer activity of coumarin derivatives. European Journal of Medicinal Chemistry Reports2022,5:100038.
7 Chakraborty R, Roy S. Angiotensin?converting enzyme inhibitors from plants:A review of their diversity,modes of action,prospects,and concerns in the management of diabetes?centric complications. Journal of Integrative Medicine202119(6):478-492.
8 Zhao M, Liu Z G, Zhang H X,et al. Germination characteristics is more associated with phylogeny?related traits of species in a salinized grassland of northeastern China. Frontiers in Ecology and Evolution2021,9:748038.
9 Li J, Bai Y C, Xie Y L,et al. Ultrastructure change and transcriptome analysis of GA3 treatment on seed germination of moso bamboo (Phyllostachys edulis). Plant Signaling & Behavior202217(1):2091305.
10 Lv Y, Pan J J, Wang H P,et al. Melatonin inhibits seed germination by crosstalk with abscisic acid,gibberellin,and auxin in Arabidopsis. Journal of Pineal Research202170(4):e12736.
11 米永伟,龚成文,王国祥,等. 当归果翅对种子吸水与发芽进程的影响. 植物研究202141(2):174-179.
Mi Y W, Gong C W, Wang G X,et al. Responses of water absorption and germination of Angelica sinensis seeds to winged perianth removal. Bulletin of Botanical Research202141(2):174-179.
12 索文龙,周东洁,王国平,等. 外源植物生长调节剂对不同发育天数烟草种子萌发的影响. 种子202140(12):116-120.
Suo W L, Zhou D J, Wang G P,et al. Effects of exogenous plant growth regulators on seed germination of tobacco at different develop?mental days. Seed202140(12):116-120.
13 周红海,周欣悦. 水引发条件下“两段浸种法”对早稻种子发芽率和秧苗素质的影响. 中国稻米201824(1):96-97.
Zhou H H, Zhou X Y. Study of hydropriming on rice seed germination rate and seedling quality by using two?phase soaking method. China Rice201824(1):96-97.
14 崔少杰,华明艳,宋兰芳,等. 不同药剂处理对草莓种子萌发的影响. 天津农业科学202329(3):20-25.
Cui S J, Hua M Y, Song L F,et al. Effects of different regents treatments on the germination of strawberry seeds. Tianjin Agricultural Sciences202329(3):20-25.
15 王楠,蔺海明,武延安. 当归种子活力. 兰州大学学报(自然科学版)200844(3):56-59.
Wang N, Lin H M, Wu Y A. Seed germination vigor of Angelica sinensis . Journal of Lanzhou University (Natural Sciences)200844(3):56-59.
16 罗建民,刘欢,王敬龙,等. 打破野生藏沙蒿种子休眠方法的筛选与评价. 草地学报202230(6):1603-1612.
Luo J M, Liu H, Wang J L,et al. Screening and evaluation of methods for breaking seed dormancy of wild Artemisia wellbyi . Acta Agrestia Sinica202230(6):1603-1612.
17 王宁,付亚军,袁美丽,等. GA3浸种对入侵植物节节麦种子破眠及发芽特性的影响. 草业学报202029(2):73-81.
Wang N, Fu Y J, Yuan M L,et al. Effectiveness of exogenous GA3 for dormancy breaking in invasive Aegilops tauschii,and effect on germination physiology. Acta Prataculturae Sinica202029(2):73-81.
18 梁佳,马依努·吾斯曼,方志刚. 外源生长物质对干旱胁迫条件下甜高粱种子萌发的影响. 草地学报202129(3):610-617.
Liang J, Mayinu W, Fang Z G,Effects of exogenous growth substances on seed germination of sweet sorghum under drought stress. Acta Agrestia Sinica202129(3):610-617.
19 胡亚丽,聂靖芝,吴霞,等. 水杨酸引发对红麻幼苗耐盐性的影响. 中国农业科学202255(14):2696-2708.
Hu Y L, Nie J Z, Wu X,et al. Effect of salicylic acid priming on salt tolerance of Kenaf seedlings. Scientia Agricultura Sinica202255(14):2696-2708.
20 吴锋,高国训,王武台,等. 不同浓度6?BA包衣芹菜种子对萌发效果的影响. 天津农业科学201521(8):139-143.
Wu F, Gao G X, Wang W T,et al. Effect of different concentration 6?BA coated celery seed on germination. Tianjin Agricultural Sciences201521(8):139-143.
21 刘桂婷. 药食两用植物——明日叶栽培技术研究. 农业开发与装备2013(1):69.
22 昌正兴,郭玉姣,王安华. 冬瓜种子发芽率提高方法探究. 南方农业202115(29):11-13,35.
23 陈子敬,于茜,丁云玉,等. 种子引发对芹菜种子发芽、幼苗生长及产量的影响. 种子201635(4):26-29.
Chen Z J, Yu Q, Ding Y Y,et al. The effect of seed priming on celery seed germination,seedling growth and yield. Seed201635(4):26-29.
24 谭杉杉,仇亮,段奥其,等. 次氯酸钠处理种子对芹菜幼苗可溶性糖含量及相关基因表达的影响. 植物生理学报202258(1):165-172.
Tan S S, Qiu L, Duan A Q,et al. Effects of seed?soaking with sodium hypochlorite on soluble sugar content and expression profiles of related genes of celery seedlings. Plant Physiology Journal202258(1):165-172.
25 Thongtip A, Mosaleeyanon K, Korinsak S,et al. Promotion of seed germination and early plant growth by KNO3 and light spectra in Ocimum tenuiflorum using a plant factory. Scientific Reports202212(1):6995.
26 Anfang M, Shani E. Transport mechanisms of plant hormones. Current Opinion in Plant Biology2021,63:102055.
27 禄亚洲,魏若晨,李宁,等. 不同消毒剂对濒危药材西藏龙胆种子的消毒效果及萌发的影响. 种子201736(9):81-83.
Lu Y Z, Wei R C, Li N,et al. Effects of different disinfectants on sterilization and germination of Gentiana tibetica King ex Hook.f. Seed201736(9):81-83.
28 Buijs G. A perspective on secondary seed dormancy in Arabidopsis thaliana . Plants20209(6):749.
29 周玉雷,成启明,都帅,等. 不同处理方法对紫花苜蓿种子萌发的影响. 中国饲料2020(14):16-23.
Zhou Y L, Cheng Q M, Du S,et al. Effect of different treatment methods on alfalfa seed germination. China Feed2020(14):16-23.
30 Chen X C, Yoong F Y, O'neill C M,et al. Temperature during seed maturation controls seed vigour through ABA breakdown in the endosperm and causes a passive effect on DOG1 mRNA levels during entry into quiescence. New Phytologist2021232(3):1311-1322.
31 梁润芳,武自念,李志勇,等. 低温和光照对羊草种子萌发的影响. 中国草地学报202143(12):33-39.
Liang R F, Wu Z N, Li Z Y,et al. Effects of low?temperature and light on seed germination of Leymus chinensis . Chinese Journal of Grassland202143(12):33-39.
32 Rizk T Y, Kholousy A S O, Saudy H S,et al. Breaking dormancy and enhancing germination of Avena sterilis L. and Amaranthus retroflexus L. weeds by gibberellic acid and potassium nitrate to keep soil and crops healthy. Gesunde Pflanzen202275(4):757-763.
33 刘镎,王荣华,王维成,等. 硼酸和硝酸钾引发甜菜种子的研究. 黑龙江大学自然科学学报202239(6):686-691.
Liu N, Wang R H, Wang W C,et al. Study on seed initiation of sugar beet by boric acid and potassium nitrate. Journal of Natural Science of Heilongjiang University202239(6):686-691.
34 Wang Y, Zhang J, He W,et al. Effect of gibberellin treatment on dormancy?breaking and germination of cherry Seeds. IOP Conference Series:Earth and Environmental Science2020,446:032079.
35 Hosseini M S, Zahedi S M, Hoveizeh N F,et al. Improving seed germination and seedling growth of guava under heat and osmotic stresses by chemical and hormonal seed treatments. Bragantia202079(4):387-399.
36 殷武平,袁祖华,彭莹,等. 不同浸种和催芽处理对芹菜种子发芽的影响. 中国瓜菜202134(11):100-103.
Yin W P, Yuan Z H, Peng Y,et al. Effects of different seed soaking and germination treatments on the germination of celery seeds. China Cucurbits and Vegetables202134(11):100-103.
37 Chu C G, Poore R C, Bolton M D,et al. Mechanism of sugarbeet seed germination enhanced by hydrogen peroxide. Frontiers in Plant Science2022,13:888519.
[1] 仲昭朝, 邹 婷, 唐惠炜, 庄 重, 张 臻. 铜胁迫对蚕豆根尖细胞凋亡及线粒体功能的影响[J]. 南京大学学报(自然科学版), 2019, 55(1): 154-160.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!