ADS1298 and ADS1252 are two wonderful ICs issued by TI company, which can be used for bioelectricity acquisition system. In this paper, the mechanisms of sampling configuration of the two ICs are summarized first. For ADS1298 configuration, there are two first-time sampling modes to be selected, one is high-precision mode with the first-time sampling rate 512 kHz, and the other is the low-power mode with its first-time sampling rate 256 kHz; then a low-pass filter is configured with an order parameter N, and its frequency response is of SINC type function. For ADS1252, the external clock is divided by 6, getting the first-time sample rate, and then also a SINC low-pass filter is used with a fixed order parameter 64. After filtering, for both ICs, a resampling process with certain rate is performed, and then the final data are output to us for collection. On basis of these, the specific sampling configuration schemes for typical ECG acquisition systems with the two ICs are proposed, and the corresponding performance are simulated. ADS1298 is configured to a high-precision mode, its resampling parameter N is configured with 1024, and ADS1252’s system clock is set as 0.192 MHz, so that both ICs can get the same final sampling rate of 500 Hz. The paper also built a real typical ECG acquisition system with ADS 1298 and C8051F320 configured using the proposed scheme, which has been able to acquire the real ECG signals. After test, basically, the amplitude frequency response of the real system is consistent with the simulated result. It is believed that this work is useful for the sampling configuration of the two ICs when used in bioelectricity acquisition applications