The over-healing is a major problem in the process of bile duct injury repair, It will cause malignant out comes because of stricture by scar formation, It is necessary to promote rapid-healing。[the injury to prevent scar formation, In our previous work，we produced a sustained-release basic fibroblast growth factor(bFUF). After adding to collagen membrane it could not only retain the activity of growth factor but also obtain the ability of sustained and site-targeting.ln our study,we used this material to repair a 2. 0 X 0. 5cm elliptical defect in the bile duct of pigs,and blank collagen membranes with same size were used as control. Before being sacrificed，the alanine aminotransferase (ALT)，y-glutamyltransferase(GGT),direct bilirubin(DBil)，and total bile acid(TBA) in both groups wrere detected and compared. After being sacrificed，the H & E staining and Masson’s trichrome staining of the neo-bile duct and the immunohistochemical staining of crSMA in both groups was observed and counted，the anti-crSMA antibody was used to mark the myofibroblast(MFB)which is an important factor in the process of scar formation.The cholangiog- raphy was performed under fluoroscopic guidance by cholecystostomy to evaluate if there was any evidence of strir
ture formation at 6 months. Hg-E staining showed the faster healing rate of nco-bile duct and less scar formation af- to treated by sustained-release bFUF, and the thickness of nco-bile duct was similar with normal bile duct at 3 weeks. Compared with control group,thc levels of GGT and TBA were significant low at all time points;immunohis- tochemical staining showed the expression of MFB was also significant low at all time points.The structure of colla- gen fibers was similar with the normal bile duct as showed by Masson’s trichrome staining, It is proved that using a sustained-release bFGF could effectively prevent postoperative bile duct scar formation by accelerating the healing rate of bile duct and dowrrregulating the expression of MFB.
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