安徽铜陵酸性矿山排水中霉菌TY6－2的生物矿化作用实验研究

南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (5): 841–.

安徽铜陵酸性矿山排水中霉菌TY6－2的生物矿化作用实验研究

黄　璐1，梁文英2，严　畅3，李健慧1，李文琦1，陆现彩4，李　娟4，王睿勇1*

出版日期:2017-09-25 发布日期:2017-09-25
作者简介: 1.南京大学生命科学学院，南京，210023；2.南京大学金陵学院化学与生命科学学院，南京，210089；3．南京师范大学附属中学，南京，210003；4.南京大学地球科学与工程学院，南京，210023
基金资助:
基金项目：国家重点基础研究发展计划(973计划)(2014CB846004)，国家自然科学基金(41425009)
收稿日期：2017－05－24
*通讯联系人，E－mail：wangry@nju.edu.cn

A study on the biomineralizition by TY6－2，a fungus isolated from acid mine environment in Tongling，Anhui Province

Huang Lu1，Liang Wenying2，Yan Chang3，Li Jianhui1，Li Wenqi1，Lu Xiancai4，Li Juan4，Wang Ruiyong1*

Online:2017-09-25 Published:2017-09-25
About author: 1.School of Life Science，Nanjing University，Nanjing，210023，China；
2．School of Chemistry and Life Science，School of Jinling，Nanjing University，Nanjing，210089，China；
3．High School Affiliated to Nanjing Normal University，Nanjing，210003，China；
4．School of Earth Science and Engineering，Nanjing University，Nanjing，210023，China

摘要/Abstract

摘要： 从安徽铜陵狮子山矿区采集酸矿水、底泥及尾砂样品，采用YE，Fe，FeT，FeS四种选择性培养基，分离获得原核微生物17株，真核微生物50株．其中一株霉菌TY6－2经鉴定为草酸青霉Penicillium oxalicum.TY6－2在近中性环境中能够吸附铁离子，吸附率高达72%，相同条件下吸附效率显著高于分离到的其他霉菌．对TY6－2与培养液长期相互作用形成的沉淀物进行分析，发现沉淀物主要以三价铁矿物为主．TY6－2活菌生物成矿能力高于灭活处理的死菌，活菌菌体表面有大量矿物颗粒附着，且形态规则．能谱分析表明很可能是黄钠铁钒．研究表明TY6－2的生物吸附和生物成矿特性，对酸矿水的生物修复和铁的回收再利用具有重要价值．

Abstract: 17 strains of prokaryotic microorganisms and 50 strains of eukaryotic microorganisms were isolated from the acid mine drainage water，sediments and tailings samples collected from Shizishan sulfide mine，Tongling，Anhui Province with four selective media YE，Fe，FeT，FeS.Among them a fungal strain of TY6－2 was identified as Penicillium oxalicum.TY6－2 has the ability to absorb iron ions in near neutral environment and the adsorption rate is as high as 72%，obviously higher than that of the other isolated fungi.When TY6－2 interacted with culture liquid for long－term，precipitations were formed；and the mineral precipitations were mainly composed of ferric mineral.Biomineralizition ability of TY6－2 live cells is higher than that of dead cells with sterilization treatment.A large number of mineral particles which is more regular attached to live cell surfaces.The energy spectrum analysis shows that is likely to be sodium jarosite.The research on biological adsorption and biomineralization characteristics of TY6－2 is not only benefit to bioremediation of acid mine environment，but also has important value for the recovery of iron from acid mine water，sediment and tailings.

黄　璐1，梁文英2，严　畅3，李健慧1，李文琦1，陆现彩4，李　娟4，王睿勇1*. 安徽铜陵酸性矿山排水中霉菌TY6－2的生物矿化作用实验研究[J]. 南京大学学报(自然科学版), 2017, 53(5): 841–.

Huang Lu1，Liang Wenying2，Yan Chang3，Li Jianhui1，Li Wenqi1，Lu Xiancai4，Li Juan4，Wang Ruiyong1*. A study on the biomineralizition by TY6－2，a fungus isolated from acid mine environment in Tongling，Anhui Province[J]. Journal of Nanjing University(Natural Sciences), 2017, 53(5): 841–.

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