南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (2): 309.
路俊玲,彭宇科,陈 旭,肖 琳*
Lu Junling,Peng Yuke,Chen Xu,Xiao Lin*
摘要: 在水体的原位修复中,微生物的活性常受到温度、pH以及金属离子等的影响,因此强化及保持微生物的活性在实际工程应用中具有重要意义.以海藻酸钠为载体,对邻苯二甲酸酯降解菌M11进行固定化,并对比了固定化与非固定化邻苯二甲酸酯降解菌M11对邻苯二甲酸酯的降解能力.结果表明,同等条件下固定化M11对底物的降解性能明显优于游离态;以无机盐培养基作为其降解体系,反应温度控制在50 ℃,pH为8.0时,底物降解性能最优.固定化M11对底物邻苯二甲酸二丁酯的降解符合一阶动力学方程,且对底物浓度有一定的耐受范围;固定化M11的环境耐受性大大增强,反复利用性能良好.
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