南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (2): 309–.

• • 上一篇    下一篇

包埋法固定化对邻苯二甲酸酯降解菌功能的影响

路俊玲,彭宇科,陈 旭,肖 琳*   

  • 出版日期:2017-03-26 发布日期:2017-03-26
  • 作者简介:南京大学环境学院,污染控制与资源化国家重点实验室,南京,210023
  • 基金资助:
    基金项目:国家重点研发计划(2016YFC0502801)收稿日期:2016-11-23*通讯联系人,E-mail:xiaolin@nju.edu.cn

Effects of immobilization on the activity of the phthalic acid esters degradation bacteria

Lu Junling,Peng Yuke,Chen Xu,Xiao Lin*   

  • Online:2017-03-26 Published:2017-03-26
  • About author:State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing,210023,China

摘要: 在水体的原位修复中,微生物的活性常受到温度、pH以及金属离子等的影响,因此强化及保持微生物的活性在实际工程应用中具有重要意义.以海藻酸钠为载体,对邻苯二甲酸酯降解菌M11进行固定化,并对比了固定化与非固定化邻苯二甲酸酯降解菌M11对邻苯二甲酸酯的降解能力.结果表明,同等条件下固定化M11对底物的降解性能明显优于游离态;以无机盐培养基作为其降解体系,反应温度控制在50 ℃,pH为8.0时,底物降解性能最优.固定化M11对底物邻苯二甲酸二丁酯的降解符合一阶动力学方程,且对底物浓度有一定的耐受范围;固定化M11的环境耐受性大大增强,反复利用性能良好.

Abstract: The activity of microorganism in water in?situ remediation is influenced by temperature,pH and the metal ion,so strengthen and maintain the activity of system has a great significance for the practical engineering application.The article immobilized the high efficient degradation bacteria M11 of the phthalic acid esters with the sodium alginate as a carrier and compared the degradation rate of the immobilized and free M11.The result revealed that the degradation rate of immobilized M11 are much better than free M11 under the same conditions.The immobilized M11 achieved the highest removal rate with 50 ℃,pH 8.0,a certain amount of the inorganic salt medium as its degradation system.The immobilized M11 had a certain tolerance range for the concentration of di?n?butyl phthalate(DBP),of which the degradation was in conformity with the first?order kinetics equation.Sodium alginate could protect the M11,which greatly enhanced the environment tolerance.In addition,immobilized M11 could be used repeatedly for a long time.

[1] Piersma A H,Verhoef A,te Biesebeek J D,et al.Developmental toxicity of butyl benzyl phthalate in the rat using a multiple dose study design.Reproductive Toxicology,2000,14(5):417-425.[2] 熊叶丹,万树青.水体中环境激素的种类与危害.广州化工,2010(1):167-168.(Xiong Y D,Wan S Q.The Species and the Detriment of Environmental Hormones in the Aquatic System.Guangzhou Chemical Industry,2010(1):167-168.)[3] 滕玉洁,王幸丹,崔崇威.环境激素的种类及危害分析.环境科学与管理,2008,33(6):20-23.(Teng Y J,Wang X D,Cui C W.Harmfulness analysis and species on environmental hormones.Environmental Science and Management,2008,33(6):20-23.)[4] 万 洋.腐殖酸对邻苯二甲酸二丁酯的吸附、降解影响特征研究.硕士学位论文.重庆:西南大学,2012.(Wan Y.Study on Adsorption?Desorption Characteristics of Dibutyl?phthalate on humic acid.Master Dissertation.Chongqing:Southwest University,2012.)[5] Genschow E,Scholz G,Brown N,et al.Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study.In Vitro & Molecular Toxicology,2000,13(1):51-66.[6] Staples C A,Adams W J,Parkerton T F,et al.Aquatic toxicity of eighteen phthalate esters.Environmental Toxicology and Chemistry,1997,16(5):875-891.[7] Chen X,Zhang X,Yang Y,et al.Biodegradation of an endocrine?disrupting chemical di?n?butyl phthalate by newly isolated Camelimonas sp.and enzymatic properties of its hydrolase.Biodegradation,2015,26(2):171-82.[8] 王建龙.生物固定化技术与水污染控制.北京:科学出版社,2002.(Wang J L.Microbial Imm obilization Techniques and Water Pollution Control.Beijing:Science Press,2002)[9] 翟晓萌,李道棠.海藻酸钠固定化包埋微生物处理有机微污染源水.环境科学,2000,21(6):80-84.(Zhai X M,Li D T.Organic Micropolluted Source Water Treatment by Immobilized Cells.Environmental Science,2000,21(6):80-84.)[10] Tallur P N,Megadi V B,Ninnekar H Z.Biodegradation of p?cresol by immobilized cells of Bacillus sp.strain PHN 1.Biodegradation,2009,20(1):79-83.[11] 周运听.固定化细菌降解苯酚的研究.江西科学,2008,26(4):572-575.(Zhou Y T.Study on the Phenol?degrading by Immobilized Bacterium.Jiangxi Science,2008,26(4):572-575.)[12] 叶正芳,倪晋仁.污水处理的固定化微生物与游离微生物性能的比较.应用基础与工程科学学报,2002,10(4):325-331.(Ye Z F,Ni J R.Performance Comparison between the Immobilized and the Dissociated Microorganisms in Wastewater Treatment.Journal of Basic Science and Engineering,2002,10(4):325-331.)[13] 曾 锋,傅家谟,盛国英等.邻苯二甲酸二丁酯的微生物降解.环境科学,1999(5):49-51.(Zeng F,Fu J M,Sheng G Y,et al.Biodegradation of Di?n?butyl Phthalate.Environmental Science,1999(5):49-51.)[14] 李青云,周茂钟,刘幽燕等.固定化铜绿假单胞菌GF31对氯氰菊酯降解的强化作用.化工学报,2013,64(6):2219-2226.(Li Q Y,Zhou M Z,Liu Y Y,et al.Bioaugmentation strategy to enhance cypermethrin degredation by immobilized Pseudomonas GF31.Journal of Chemical Industry and Engineering,2013,64(6):2219-2226.)[15] 郑永良,肖 婷,钟玉林等.一株酚降解菌株的分离鉴定及特性研究.湖北农业科学,2010,49(9):2097-2100.(Zheng Y L,Xiao T,Zhong Y L,et al.Isolation and Identification of A Phenol?Degradation Strain and Its Characteristics Analysis.Hubei Agricultural Science,2010,49(9):2097-2100.)[16] Monteiro á A M G,Boaventura R A R,Rodrigues A E.Phenol biodegradation by Pseudomonas putida DSM 548 in a batch reactor.Biochemical Engineering Journal,2000,6(1):45-49.[17] 李超敏,韩梅,张 良等.细胞固定化技术—海藻酸钠包埋法的研究进展.安徽农业科学,2006,34(7):1281-1282.(Li C M,Han M,Zhang L,et al.Development of the sodium?aliginate immobilized cell technology.Journal of Anhui Agricultural Science,2006,34(7):1281-1282.)
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!