不同浓度砷酸盐胁迫对蜈蚣草根际微生物群落功能多样性特征的影响

南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (2): 275–.

不同浓度砷酸盐胁迫对蜈蚣草根际微生物群落功能多样性特征的影响

韩永和，贾梦茹，傅景威，向　萍，史孝霞，崔昕毅，罗　军，陈焱山*

出版日期:2017-03-26 发布日期:2017-03-26
作者简介:南京大学环境学院，污染控制与资源化国家重点实验室，南京，210023
基金资助:
基金项目：江苏省博士研究生创新项目(KYLX_0052)，南京大学优秀博士研究生创新能力提升计划B(201601B010) 收稿日期：2016－11－12 *通讯联系人，E-mail：chenyanshan@nju.edu.cn

Impacts of arsenate concentrations on functional diversities of rhizosphere microbial communities of Pteris vittata

Han Yonghe，Jia Mengru，Fu Jingwei，Xiang Ping，Shi Xiaoxia，Cui Xinyi，Luo Jun，Chen Yanshan*

Online:2017-03-26 Published:2017-03-26
About author:State Key Laboratory of Pollution Control and Resource Reuse，School of the Environment， Nanjing University，Nanjing，210023，China

摘要/Abstract

摘要： 蜈蚣草是世界上第一种被发现的砷超富集植物，其根际微生物对土壤砷的转化及蜈蚣草砷的吸收具有重要影响．采用qPCR和Biolog?Eco微平板法对砷胁迫下蜈蚣草根际微生物群落功能多样性特征的差异进行了研究．首先对蜈蚣草根际微生物的16S rRNA基因丰度进行定量分析；其次，利用Biolog?Eco微平板法分析微生物对31种碳源的平均利用率；同时，通过主成分分析、Shannon多样性指数、Pielou均匀度指数和Simpson优势度指数等探讨了微生物对不同类型碳源利用的差异和砷胁迫对微生物多样性的影响．结果表明，砷胁迫不仅降低16S rRNA基因丰度、减少微生物数量，还会显著影响微生物对碳源的利用效率．其中，微生物对碳水化合物类、氨基酸类和胺类碳源的利用受砷胁迫的影响最显著．此外，砷胁迫会诱导抗砷微生物成为优势群落，结构趋于单一．由于长期暴露可增加蜈蚣草根际微生物的数量，后期研究可深入探讨蜈蚣草根系分泌物中特异碳源物质在强化微生物－蜈蚣草修复砷污染土壤过程中的作用．

Abstract: Pteris vittata is the first reported arsenic(As)hyperaccumulator，and the rhizosphere microorganisms may play important roles in As transformation in the soils and As uptake by the plants.The aim of this work was to investigate the impacts of arsenate(As(V))concentration stress on functional diversities of microbial communities in rhizosphere of Pteris vittata，using qPCR and Biolog?Eco microplate methods.Qualitative analyses of the microbial 16S rRNA gene abundance in P．vittata rhizosphere were used to evaluate the effects of As stress on microbial population change.To test the relationships between the microbial community functional changes and As(V) concentrations，Biolog?Eco microplate?based method was used to analyze the average well color development(AWCD)for the thirty?one carbon sources by inoculating microorganisms.Besides，the principal component analysis，Shannon diversity index，Pielou evenness index and Simpson dominance index were also used to investigate the difference in carbon source utilization by microorganisms and the impacts of As(V) concentration stress on microbial diversity.Results showed that As stress not only decreased 16S rRNA gene abundance and decreased microbial population，but also significantly affected carbon utilization.Among different carbon sources，carbohydrate?，amino acid?，and amine?type carbon resources were inhibited more significantly by As than others，indicating that the microbial adaption to As stress was achieved by the cost of reducing the consumption of carbohydrates，amino acids and amines.Moreover，As stress decreased the Shannon diversity index，Pielou evenness index and Simpson dominance index of the microorganisms colonizing the P．vittata rhizosphere.This indicated that the As?resistant microorganisms might dominate the diversity?reduced microbial communities，highlighting their roles in As transformation in the rhizosphere of P．vittata.Since a long?term exposure could increase the population of rhizospheric microorganisms，further studies should focus mostly on the roles and mechanisms of the exudates of P．vittata in regulating the microbial community structure and functional diversity.Our data also shed light on improving P．vittata?mediated phytoremediation effect of As?contaminated soils，probably by applying those carbon resources in the exudates to enhance microorganisms’ activity.

韩永和，贾梦茹，傅景威，向　萍，史孝霞，崔昕毅，罗　军，陈焱山*. 不同浓度砷酸盐胁迫对蜈蚣草根际微生物群落功能多样性特征的影响[J]. 南京大学学报(自然科学版), 2017, 53(2): 275–.

Han Yonghe，Jia Mengru，Fu Jingwei，Xiang Ping，Shi Xiaoxia，Cui Xinyi，Luo Jun，Chen Yanshan*. Impacts of arsenate concentrations on functional diversities of rhizosphere microbial communities of Pteris vittata[J]. Journal of Nanjing University(Natural Sciences), 2017, 53(2): 275–.

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