南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (2): 218–.

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四溴双酚A(TBBPA)好氧降解菌Pseudomonas sp.JDT 的筛选及其对TBBPA的降解转化

 赵莹莹,王永峰,孙棐斐,郭红岩,季 荣*   

  • 发布日期:2017-03-25
  • 作者简介:?南京大学环境学院污染控制与资源化国家重点实验室,南京,210023
  • 基金资助:
    ?基金项目:国家自然科学基金(21237001)
    收稿日期:2016-07-03
    *通讯联系人,E-mail:ji@nju.edu.cn

Aerobic degradation of Tetrabranobisphenol A(TBBPA) by Pseudomonas sp.JDT isolated from an activated sludge

 Zhao Yingying,Wang Yongfeng,Sun Feifei,Guo Hongyan,Ji Rong*   

  • Published:2017-03-25
  • About author:?State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment, Nanjing University,Nanjing,210023,China

摘要:  四溴双酚A(TBBPA)作为一种被广泛使用的溴代阻燃剂,在环境中广泛存在.从活性污泥中筛选出一株TBBPA的好氧降解菌,经16S rDNA测序鉴定为假单胞菌属,命名为Pseudomonas sp.JDT.该菌在40天内对TBBPA的脱溴率为12.9%.外加碳源能够促进该菌对TBBPA的矿化,且低浓度酵母提取物的促进作用高于葡萄糖和高浓度酵母提取物.利用14C示踪技术,在持续加有外来碳源的纯菌培养体系中测定JDT对TBBPA的降解,发现18.5天后TBBPA的矿化率为2.7%,并检测到两种极性代谢产物,分别占有机可提取部分总放射性量的11.2%和47.2%.Pseudomonas sp.JDT可以作为研究环境中TBBPA微生物降解途径的实验材料.

关键词: 四溴双酚A, 微生物降解, 假单胞菌属, 14C示踪技术

Abstract:  A TBBPA-degrading aerobic bacterial strain was isolated from an activated sludge and was identified as a member of the genus Pseudomonas according to 16S rDNA gene analysis.The strain was named as Pseudomonas sp.JDT.After 40-day incubation,12.9% of TBBPA was debrominated by Pseudomonas sp.JDT.Extra carbon addition enhanced TBBPA mineralization by JDT.The inductive effects from yeast extract at a lower concentration was higher than that at a higher concentration.In the presence of extra carbon sources,2.7% of initial TBBPA was mineralized after 18.5 days incubation.Two metabolites with a higher polarity than TBBPA were detected at the end of incubation,accounting for 11.2% and 47.2% of extractable radioactivity,respectively.The results suggest that Pseudomonas sp.JDT may be used for study on the biodegradation pathway of TBBPA in the environment.

Key words: TBBPA, microbial degradation, Pseudomonas, 14C tracer

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