四溴双酚A(TBBPA)好氧降解菌Pseudomonas sp.JDT 的筛选及其对TBBPA的降解转化

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南京大学学报(自然科学版) ›› 2017, Vol. 53 ›› Issue (2) : 218.

赵莹莹，王永峰，孙棐斐，郭红岩，季　荣*

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Aerobic degradation of Tetrabranobisphenol A(TBBPA) by Pseudomonas sp.JDT isolated from an activated sludge

Zhao Yingying，Wang Yongfeng，Sun Feifei，Guo Hongyan，Ji Rong*

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摘要

四溴双酚A(TBBPA)作为一种被广泛使用的溴代阻燃剂，在环境中广泛存在．从活性污泥中筛选出一株TBBPA的好氧降解菌，经16S rDNA测序鉴定为假单胞菌属，命名为Pseudomonas sp.JDT.该菌在40天内对TBBPA的脱溴率为12.9%.外加碳源能够促进该菌对TBBPA的矿化，且低浓度酵母提取物的促进作用高于葡萄糖和高浓度酵母提取物．利用14C示踪技术，在持续加有外来碳源的纯菌培养体系中测定JDT对TBBPA的降解，发现18.5天后TBBPA的矿化率为2.7%，并检测到两种极性代谢产物，分别占有机可提取部分总放射性量的11.2%和47.2%.Pseudomonas sp.JDT可以作为研究环境中TBBPA微生物降解途径的实验材料．

Abstract

A TBBPA-degrading aerobic bacterial strain was isolated from an activated sludge and was identified as a member of the genus Pseudomonas according to 16S rDNA gene analysis.The strain was named as Pseudomonas sp.JDT.After 40-day incubation，12.9% of TBBPA was debrominated by Pseudomonas sp.JDT.Extra carbon addition enhanced TBBPA mineralization by JDT.The inductive effects from yeast extract at a lower concentration was higher than that at a higher concentration.In the presence of extra carbon sources，2.7% of initial TBBPA was mineralized after 18.5 days incubation.Two metabolites with a higher polarity than TBBPA were detected at the end of incubation，accounting for 11.2% and 47.2% of extractable radioactivity，respectively.The results suggest that Pseudomonas sp.JDT may be used for study on the biodegradation pathway of TBBPA in the environment.

导出引用

赵莹莹, 王永峰, 孙棐斐, 郭红岩, 季　荣. 四溴双酚A(TBBPA)好氧降解菌Pseudomonas sp.JDT 的筛选及其对TBBPA的降解转化[J]. 南京大学学报(自然科学版), 2017, 53(2): 218

Zhao Yingying, Wang Yongfeng, Sun Feifei, Guo Hongyan, Ji Rong. Aerobic degradation of Tetrabranobisphenol A(TBBPA) by Pseudomonas sp.JDT isolated from an activated sludge[J]. Journal of Nanjing University(Natural Sciences), 2017, 53(2): 218

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