To investigate the bioaccumulation of carbonbased nanomaterial，we synthesized the 14C-labeled carbon dots(Cdots)by oneway microwave pyrolysis.Two marine algae，Prorocentrum micans and Prorocentrum donghaiense were used as the objective organisms，and their accumulation of Cdots was compared.As the 14C signal from the Cdots may be quenched inside the cells，the results of the different ultrasonicating methods were compared，and the counting number was the highest when the cells were ultrasonicated for 5 minutes.After the optimization of the cell processing methods，we further explored the bioaccumulation kinetics of Cdots by both algae and the accumulation results with different units(pg?cell－1，pg?μm－2，and pg?μm－3)were compared.As expected，cellular concentration of Cdots increased linearly with exposure time regardless of the units of the accumulation results.Nevertheless，when the accumulation results were expressed in the unit of pg?μm－3，potential accumulation difference between the two algae disappeared and this unit would be used in the bioaccumulation studies of Cdots in the future.
Li Jiameng，Yang Liuyan，Miao Aijun*.
The synthesis of 14C-labelled carbon dots and their accumulation in marine phytoplankton [J]. Journal of Nanjing University(Natural Sciences), 2016, 52(3): 512-519
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Navarro E，Baun A，Behra R，et al.Environmental behavior and ecotoxicity of engineered nanoparticles to algae，plants，and fungi.Ecotoxicology，2008，17(5)：372－386. [2] Gwinn M R，Vallyathan V．Nanoparticles：Health effectspros and cons.Environmental Health Perspectives，2006，1818－1825. [3] Wang Y，Miao A J，Luo J，et al.Bioaccumulation of CdTe quantum dots in a freshwater alga Ochromonas danica：A kinetics study.Environmental Science & Technology，2013，47(18)：10601－10610. [4] Li J，Strong R，Trevisan J，et al.Doserelated alterations of carbon nanoparticles in mammalian cells detected using biospectroscopy：Potential for realworld effects.Environmental Science & Technology，2013，47(17)：10005－10011. [5] Liu Q，Guo B，Rao Z，et al.Strong twophotoninduced fluorescence from photostable，biocompatible nitrogendoped graphene quantum dots for cellular and deeptissue imaging.Nano Letters，2013，13(6)：2436－2441. [6] Shen R，Song K，Liu H，et al.Fluorescence enhancement and radiolysis of carbon dots through aqueous γ radiation chemistry.The Journal of Physical Chemistry C，2012，116(29)：15826－15832. [7] Czarny B，Georgin D，Berthon F，et al.Carbon nanotube translocation to distant organs after pulmonary exposure：Insights from in situ 14Cradiolabeling and tissue radioimaging.ACS Nano，2014，8(6)：5715－5724. [8] Guo X，Dong S，Petersen E J，et al.Biological uptake and depuration of radiolabeled graphene by Daphnia magna.Environmental Science & Technology，2013，47(21)：12524－12531. [9] Li D，Fortner J D，Johnson D R，et al.Bioaccumulation of 14C60 by the earthworm Eisenia fetida.Environmental Science & Technology，2010，44(23)：9170－9175. [10] Petersen E J，Huang Q，Weber W J．Relevance of octanolwater distribution measurements to the potential ecological uptake of multiwalled carbon nanotubes.Environmental Toxicology and Chemistry，2010，29(5)：1106－1112. [11] Zhu H，Wang X，Li Y，et al.Microwave synthesis of fluorescent carbon nanoparticles with electrochemiluminescence properties.Chemical Communications，2009(34)：5118－5120. [12] Domingos R F，Baalousha M A，JuNam Y，et al.Characterizing manufactured nanoparticles in the environment：multimethod determination of particle sizes.Environmental Science & Technology，2009，43(19)：7277－7284. [13] Guillard R R L．Culture of phytoplankton for feeding marine invertebrates.Culture of marine invertebrate animals.Springer US，1975：29－60. [14] Guillard R R L，Ryther J H．Studies of marine planktonic diatoms：I.Cyclotella nana Hustedt，and Detonula confervacea(Cleve)Gran.Canadian Journal of Microbiology，1962，8(2)：229－239. [15] Baker S N，Baker G A．Luminescent carbon nanodots：Emergent nanolights.Angewandte Chemie International Edition，2010，49(38)：6726－6744. [16] Zhang B，Liu C，Liu Y．A Novel Onestep approach to synthesize fluorescent carbon nanoparticles.European Journal of Inorganic Chemistry，2010，2010(28)：4411－4414. [17] Zhang J，Shen W，Pan D，et al.Controlled synthesis of green and blue luminescent carbon nanoparticles with high yields by the carbonization of sucrose.New Journal of Chemistry，2010，34(4)：591－593. [18] Glibert P M，Burkholder J A M，Kana T M，et al.Grazing by Karenia brevis on Synechococcus enhances its growth rate and may help to sustain blooms.Aquatic Microbial Ecology，2009，55(1)：17－30. [19] Jeong H J，Du Yoo Y，Kim J S，et al.Growth，feeding and ecological roles of the mixotrophic and heterotrophic dinoflagellates in marine planktonic food webs.Ocean Science Journal，2010，45(2)：65－91. [20] 张淑雯，欧林坚，吕颂辉等．光照及营养盐对3种赤潮甲藻吞噬营养行为的影响．海洋科学，2011，35(4)：94－99.(Zhang S W，Ou L J，Lü S H，et al.Effects of light and nutrients on the phagotrophic behaviors of three harmful dinoflagellates.Marine Science，2011，35(4)：94－99.) [21] Du Yoo Y，Jeong H J，Kim M S，et al.Feeding by Phototrophic redtide dinoflagellates on the ubiquitous marine diatom Skeletonema costatum.Journal of Eukaryotic Microbiology，2009，56(5)：413－420. [22] 张清春，于仁成，宋静静等．几株赤潮甲藻的摄食能力．生态学报，2012，32(2)：402－413.(Zhang Q C，Yu R C，Song J J，et al.Ingestion of selected HABforming dinoflagellates.Acta Ecologica Sinica，2012，32(2)：402－413.) [23] Fan C W，Reinfelder J R．Phenanthrene accumulation kinetics in marine diatoms.Environmental Science & Technology，2003，37(15)：3405－3412. [24] Tsui M T K，Wang W X．Biokinetics and tolerance development of toxic metals in Daphnia magna.Environmental Toxicology and Chemistry，2007，26(5)：1023－1032. [25] Wang W X，Fisher N S．Delineating metal accumulation pathways for marine invertebrates.Science of the Total Environment，1999，237：459－472. [26] Miao A J，Wang W X，Juneau P．Comparison of Cd，Cu，and Zn toxic effects on four marine phytoplankton by pulseamplitudemodulated fluorometry.Environmental Toxicology and Chemistry，2005，24(10)：2603－2611. [27] Chang E，Thekkek N，Yu W W，et al.Evaluation of quantum dot cytotoxicity based on intracellular uptake.Small，2006，2(12)：1412－1417. [28] Allen M J，Tung V C，Kaner R B．Honeycomb carbon：A review of graphene.Chemical Reviews，2009，110(1)：132－145. [29] Zanni E，De Bellis G，Bracciale M P，et al.Graphite nanoplatelets and Caenorhabditis elegans：Insights from an in vivo model.Nano Letters，2012，12(6)：2740－2744. [30] Zhang Y，Ali S F，Dervishi E，et al.Cytotoxicity effects of graphene and singlewall carbon nanotubes in neural phaeochromocytomaderived PC12 cells.ACS Nano，2010，4(6)：3181－3186. [31] 朱小山，朱 琳，田胜艳等．三种碳纳米材料对水生生物的毒性效应．中国环境科学，2008，28(3)：269－273.(Zhu X S，Zhu L，Tian S Y，et al.Toxicity effect of three kinds of carbonnanomaterials on aquatic organisms.China Environmental Science，2008，28(3)：269－273.)
PDF(1650583 KB)
