上覆水不同C/N比条件下沉积物环境反硝化及氨氧化功能基因丰度变化特征

南京大学学报(自然科学版) ›› 2016, Vol. 52 ›› Issue (1): 27–35.

上覆水不同C/N比条件下沉积物环境反硝化及氨氧化功能基因丰度变化特征

李筱宛1,2，张亚平1,2，万宇1,2，阮晓红1,2*

出版日期:2016-01-27 发布日期:2016-01-27
作者简介:(1. 南京大学表生地球化学教育部重点实验室,南京,210046; 2. 南京大学地球科学与工程学院,南京,210046)
基金资助:
基金项目:国家自然科学基金重点基金(41230640),国家水体污染控制与治理科技重大专项(2012ZX07204-003)
收稿日期:2015-12-10
*通讯联系人,E-mail:ruanxh@nju.edu.cn

Abundance variations of denitrification and ammonium oxidation functional genes in sediment under different C/N ratio conditions

Li Xiaowan1,2, Zhang Yaping1,2, Wan Yu1,2, Luo Yuehui1,2, Ruan Xiaohong1,2*

Online:2016-01-27 Published:2016-01-27
About author:(1. Key Laboratory of Surficial Geochemistry, Ministry of Education, Nanjing, 210046, China; 2. School of Earth Sciences and Engineering, Nanjing, 210046, China)

摘要/Abstract

摘要： 本研究以太湖梅梁湾沉积物环境为研究对象，通过室内培养试验，研究上覆水不同C/N比条件对沉积物中反硝化（nirS、nirK）及氨氧化功能基因（古菌AOA-amoA、细菌AOB-amoA）丰度的影响，实验设计硝氮水平为2.0和5.0 mg·L-1，C/N为0.5、2、4、6、10、14。结果表明，沉积物样品nirK和nirS功能基因本底丰度分别为1.54×106 copies·g-1和2.21×106 copies·g-1，氨氧化细菌和古菌功能基因本底丰度分别为1.89×103 copies·g-1和2.03×105 copies·g-1。氨氧化功能基因丰度对C/N比一定比值时，其丰度由初期的AOA-amoA）丰度对C/N比变化响应更显著。硝酸盐氮浓度2=0.551，P<0.05），当氨氧化功能基因丰度较高时，来源于沉积物中氨氮的硝化产物使得系统中硝酸盐趋于累积。

Abstract: This study investigated the relationships between C/N ratios in overlying water and abundances of denitrification and ammonium oxidation functional genes (nirK, nirS, AOA-amoA and AOB-amoA) in sediments sampled from Meiliang Bay, Taihu Lake. The experiment was conducted under two nitrate substrate concentrations of 2.0 and 5.0 mg·L-1; and the C/N ratios was selected to be 0.5、2、4、6、10、14. The results showed that, the background abundances of nirK and nirS were 1.54×106 copies·g-1 and 2.21×106 copies·g-1, respectively, those of AOB-amoA and AOA-amoA were 1.89×103 copies·g-1 and 2.03×105 copies·g-1, respectively. During 60-day incubation, the abundances of denitrification functional genes in all samples have increased, but showed no significant response to changes in C/N ratios, while the abundance of ammonium oxidation functional genes varied with the changing C/N ratios. When C/N ratio exceed a certain value, the abundance of ammonium oxidation functional genes tended to decrease after an early increase, and AOA-amoA abundance responded to the different C/N ratios more evidently. Correlation analysis indicated that the transformation rates of nitrate significantly correlated to the abundance of ammonium oxidation genes (r2=0.551, p<0.05). When the abundance of ammonium oxidation genes was at high level, nitrate tended to accumulate in the system, which might result from the nitrification of ammonia nitrogen in sediment.

李筱宛1,2，张亚平1,2，万宇1,2，阮晓红1,2*. 上覆水不同C/N比条件下沉积物环境反硝化及氨氧化功能基因丰度变化特征[J]. 南京大学学报(自然科学版), 2016, 52(1): 27–35.

Li Xiaowan1,2, Zhang Yaping1,2, Wan Yu1,2, Luo Yuehui1,2, Ruan Xiaohong1,2*. Abundance variations of denitrification and ammonium oxidation functional genes in sediment under different C/N ratio conditions[J]. Journal of Nanjing University(Natural Sciences), 2016, 52(1): 27–35.

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