纳米ZnO和微米ZnO对鲫鱼的毒性效应

南京大学学报(自然科学版) ›› 2014, Vol. 50 ›› Issue (4): 425–.

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纳米ZnO和微米ZnO对鲫鱼的毒性效应

胡正雪,尹颖,刘林,郭红岩

出版日期:2014-08-22 发布日期:2014-08-22
作者简介: 南京大学环境学院,污染控制与资源化研究国家重点实验室,南京,210023
基金资助:

Ecological toxicity of Nano-ZnO and Micro-ZnO on Carassius auratus

Hu Zhengxue, Liu Lin, Guo Hongyan, Yin Ying

Online:2014-08-22 Published:2014-08-22
About author: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China

摘要/Abstract

摘要： 以鲫鱼Carassiusauratus 为实验生物,采用电子顺磁共振等多种检测手段,比较研究了纳米和微米ZnO腹腔注射14d后,其在鲫鱼肝脏和脑部的分布情况以及毒性效应.研究发现,相同浓度的纳米和微米ZnO 作用下,纳米ZnO处理组肝脏和脑部Zn含量高于微米ZnO处理组;纳米ZnO对肝脏羟基自由基,肝脏和脑部MDA及GSH 的诱导率高于微米ZnO;纳米ZnO对肝脏和脑部SOD活性的抑制作用也高于微米ZnO.但不同浓度纳米ZnO对羟基自由基和MDA的诱导效应及对SOD活性的抑制作用并未随浓度的升高而线性增加.结果表明:相比于微米ZnO,纳米ZnO更易进入肝脏和脑部,进而产生更强的毒性效应.但纳米ZnO的毒性并没有随浓度升高而线性增加.

Abstract: Nano ZnO has attracted increasing concerns because of its widespread use and unique toxic potential. While, so far most researches focused on the concentration effects but not the size effects of Nano ZnO. The purpose of this study was to compare the toxic effects of Nano ZnO and Micro ZnO to Carassius auratus. Fourteen days after the intraperitoneal injection of Nano ZnO（1, 12.5, 50 mg ZnO/kg）and Micro ZnO (12.5 mg ZnO/kg), the distribution and toxic effects of Nano and Micro ZnO on Carassius auratus liver and brain were studied. The particle size of both Nano and Micro ZnO was determined by transmission electron microscopy (TEM). And the free radicals (˙OH) generation was determined by electron paramagnetic resonance (EPR). Several antioxidant biomarkers such as superoxide dysmutase (SOD), glutathione S-transferases (GST), glutathione (GSH) and malondialdehyde (MDA) were measured, and the effects of Nano and Micro ZnO on Zn content in liver and brain of C. auratus were also detected. We found that both Nano and Micro ZnO could induce the production of ˙OH. As compared to Micro ZnO, exposure to 12.5 mg ZnO/kg Nano ZnO caused more accumulation of ˙OH in the liver of C. auratus. Accordingly, Nano ZnO induced the synthesis of MDA and suppressed the SOD activities in the liver and brain of C. auratus. Further, Nano ZnO effects on GSH were much higher than those of Micro ZnO, suggesting more severe oxidative stress caused by Nano ZnO under the same concentration. The content of Zn in both liver and brain of C. auratus exposed to Nano ZnO was also much higher than those exposed to Micro ZnO. However, there was no linear relationship between the increased concentrations of Nano ZnO and the induction of ˙OH and MDA and the inhibition of SOD activities. Overall, the results indicated that Nano ZnO could be more effectively taken into the brain and liver of Carassius auratus than Micro ZnO, resulting in more toxic effects, but were lack of does effects with the Nano ZnO concentration.

胡正雪,尹颖,刘林,郭红岩. 纳米ZnO和微米ZnO对鲫鱼的毒性效应[J]. 南京大学学报(自然科学版), 2014, 50(4): 425–.

Hu Zhengxue, Liu Lin, Guo Hongyan, Yin Ying. Ecological toxicity of Nano-ZnO and Micro-ZnO on Carassius auratus[J]. Journal of Nanjing University(Natural Sciences), 2014, 50(4): 425–.

参考文献

[1] Cheng J, Chan C M, Veca L M, et al. Acute and long -term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio). Toxicology and Applied Pharmacology, 2008, 235(2): 216-225.
[2] Lin W, Xu Y, Huang C, et al. Toxicity of nano-and micro-sized ZnO particles in human lung epithelial cells. Journal of Nanoparticle Research, 2009, 11(1): 25-39.
[3] Gilbert B, Fakra S C, Xia T, et al. The fate of ZnO nanoparticles administered to human bronchial epithelial cells. ACS Nano, 2012, 6(6): 4921-4930.
[4] Sharma V, Singh P, Pandey A K, et al. Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles. Mutation Research, 2012, 745(1-2):84-91.
[5] Bai W, Zhang Z Y, Tian W J, et al. Toxicity of Zinc oxide nanoparticles to zebrafish embryo: A physicochemical study of toxicity mechanism. Journal of Nanoparticle Research, 2010, 12(5): 1645-1654.
[6] Ma H B, Bertsch P M, Glenn T C, et al. Toxicity of manufactured zinc oxide nanoparticles in the nematode Caenorhabditis elegans. Environmental Toxicology and Chemistry, 2009, 28(6): 1324-1330.
[7] Poynton H C, Lazorchak J M, Impellitteri C A, et al. Different gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions. Environmental Science & Technology. 2011, 45(2): 762-768.
[8] Lemaire P, Livingstone D R. Pro-oxidant/antioxidant processes and organic xenobiotic interactions in marine organisms, in particular the flounder Platichthys flesus and the mussel Mytilus edulis. Trends in Comparative Biochemistry & Physiology, 1993, 1: 1119–1150.
[9] Yin Y, Jia J, Guo H Y, et al. Pyrene-stimulated reactive oxygen species generation and oxidative damage in Carassius auratus. Journal of Environmental Science and health, Part A, 2014, 49: 162-170.
[10] Zhao L, Peng B, Hernandez-Viezcas J A, el al. Stress response and tolerance of zea mays to CeO2 Nanoparticles: Cross talk among H2O2, heat shock protein and lipid peroxidation. ACS Nano, 2012, 6(11): 9615-9622.
[11] Carvalho C S, Bernusso V A, Araujo H S S, et al. Biomarker responses as indication of contaminant effects in Oreochromis niloticus. Chemosphere, 2012, 89(1): 60-69.
[12] Shi H H, Wang X R, Luo Y, et al. Electron paramagnetic resonance evidence of hydroxyl radical generation and oxidative damage induced by tetrabromobisphenol A in Carassius auratus. Aquatic Toxicology, 2005, 74(4): 365–371.
[13] Gonzalez F B S, Repetto M, Evelson P, et al. Inhibition of microsomal lipid peroxidation by alphatocopherol and alpha-tocopherol acetate. Xenobiotica, 1991, 21(8): 1013–1022.
[14] 赵云斌, 刘敏, 余忠谊. 邻苯三酚自氧化法测定血中超氧化物歧化酶的活性. 中国卫生检验杂志, 2001, 11(4): 287-288.
[15] Habig W H, Pabst M J, Jakoby W B. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 1974, 249: 7130-7139.
[16] Hissin P J, Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical Biochemistry, 1976, 74(1): 214-226.
[17] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72(1-2): 248-254.
[18] Heng B C, Zhao X, Tan E C, et al. Evaluation of the cytotoxic and inflammatory potential of differentially shaped zinc oxide nanoparticles. Archives of Toxicology, 2011, 85: 1517–1528.
[19] Wang H, Wick R L, Xing B. Toxicity of nanoparticulate and bulk ZnO, Al2O3 and TiO2 to the nematode Caenorhabditis elegans. Environmental Pollution, 2009, 157(4): 1171-1177.
[20] 梁春柳, 朱江波, 朱玉平, 等. 纳米与微米氧化锌体外遗传毒作用特征的比较研究. 生态毒理学报, 2012, 7(3): 299-304.
[21] Song W H, Zhang J Y, Guo J, et al. Role of dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicology Letters, 2010, 199(3): 389-397.
[22] 刘伟. 几种典型纳米材料毒性与生物活性的探索. 硕士学位论文. 济南: 山东大学, 2007: 16-21.
[23] Zhang X, Yang F X, Zhang X L, et al. Induction of hepatic enzymes and oxidative stress in Chinese rare minnow (Gobiocypris rarus) exposed to waterborne hexabromocyclododecane (HBCDD). Aquatic Toxicology, 2008, 86(1): 4–11.

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