水体粪便污染的线粒体DNA 溯源研究

南京大学学报(自然科学版) ›› 2014, Vol. 50 ›› Issue (4): 388–.

水体粪便污染的线粒体DNA 溯源研究

何席伟,苗雨,陈慧梅,张徐祥

出版日期:2014-08-22 发布日期:2014-08-22
作者简介: (1.污染控制与资源化研究国家重点实验室,南京大学环境学院,南京,210023;2.南京大学医学院,南京,210093)
基金资助:
Major Science and Technology Program of China for Water Pollution Control and Treatment (2012ZX07101-002-004 and 2012ZX07506-004-004) and Environmental Protection Research Project of Jiangsu Province, China (2012044 and 2012045)

Mitochondrial DNA source tracking of water fecal contamination

He Xiwei 1, Miao Yu 1, Chen Huimei 2*, Zhang Xu-Xiang *

Online:2014-08-22 Published:2014-08-22
About author:1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China; 2. Medical School, Nanjing University, Nanjing 210093, China

摘要/Abstract

摘要： 畜禽粪便和生活污水是造成水体污染的主要因素之一.快速、准确地确定污染源对于水体污染防治尤为重要.近年来,基于线粒体DNA(mtDNA)种属特异性的污染物溯源技术已成为国际研究热点.本文综述了水体粪便污染的mtDNA溯源技术的理论基础及其应用进展,并通过与微生物溯源技术进行比较,分析了mtDNA 作为溯源标记物的优势以及存在的问题,从而为实际环境水体中粪便污染的生物溯源工作提供参考.

Abstract: Water environment is often contaminated by human and animal feces containing nutrients including carbon, nitrogen and phosphorus, as well as virulent pathogens. Correct and rapid identification of the pollution sources is considered crucial to mitigate the influence of such contamination. Mitochondrial DNA, coming directly from a host cell, is considered as an excellent genetic marker for fecal source tracking (FST) due to its high host-specificity and detection sensitivity. This paper summarizes the advances and applications of mitochondrial DNA source tracking based on comparisons with microbial source tracking, highlighting the potentials and limitations of mitochondrial DNA source tracking. As a new method, mitochondrial DNA source tracking has to be improved based on more thorough researches before it is used for routine water analysis in the future.

何席伟,苗雨,陈慧梅,张徐祥. 水体粪便污染的线粒体DNA 溯源研究[J]. 南京大学学报(自然科学版), 2014, 50(4): 388–.

He Xiwei 1, Miao Yu 1, Chen Huimei 2*, Zhang Xu-Xiang *. Mitochondrial DNA source tracking of water fecal contamination[J]. Journal of Nanjing University(Natural Sciences), 2014, 50(4): 388–.

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