两株土壤菌与蒙脱石相互作用的实验研究*

南京大学学报(自然科学版) ›› 2013, Vol. 49 ›› Issue (6): 767–.

两株土壤菌与蒙脱石相互作用的实验研究*

赵玉连1，代群威1,2**，董发勤2，蒋沁芮1，唐俊1，陈武3

出版日期:2014-01-14 发布日期:2014-01-14
作者简介:(1.西南科技大学环境与资源学院,绵阳,621010;2.固体废物处理与资源化省部共建教育重点实验室,绵阳,621010;3.西南科技大学材料科学与工程学院,绵阳,621010)
基金资助:
国家自然科学基金(41102212,41130746,11176028)

Experimental study oninteraction between two soil bacteria andsmectite

Zhao Yu-Lian1, Dai Qun-Wei1,2, Dong Fa-Qin2, Jiang Qin-Rui1,Tang Jun1, ChenWu3

Online:2014-01-14 Published:2014-01-14
About author: (1. School of Environment and Resource,Southwest University of Science and Technology, Mianyang, 621010, China; 2.Key Laboratory of Solid Waste Treatment and the Resource Recycle (SWUST, Ministry of Education), Mianyang, 621010, China; 3. School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China)

摘要/Abstract

摘要： 本文主要目的是探讨两株土壤菌与蒙脱石相互作用过程。选取了土壤中革兰氏阳性菌、革兰氏阴性菌各1株，分别与钙蒙脱石相互作用。在实验中，对葡萄糖消耗率、pH值随时间的变化趋势进行分析，发现蒙脱石对两株土壤菌的生长有一定的缓冲促进作用。蒙脱石在与土壤菌作用后，矿物晶体结构发现变化，层间距增大，C轴方向堆垛有序程序降低；FTIR分析结果表明，蒙脱石与土壤菌作用后，不仅出现了来自土壤菌的新峰，而且粘土矿物结构基团分子环境也发生改变，说明土壤菌在生长代谢产物进入蒙脱石矿物层间域内，使得蒙脱石底面间距增大。对比两株土壤菌对蒙脱石的作用效果可知，革兰氏阳性菌起着主导作用。

Abstract: This paper aims to investigate the interactionbetween two different type soil bacteria and smectite. We selected one gram-positive bacterium and one gram-negative bacterium isolated from soil to interact with Ca-smectite, respectively. In the experiment, we analyzed the variation trend of glucose (GLU) consumption rate, pH value with the time, and found that smectiteexhibited a certain buffering and promoting effect onthe growth of both bacteria. After the interaction with soil bacteria, the interlayer spacing of smectiteincreased and C-axis stacking ordered gradually decreased. In the FTIRspectra,not only a peak assigned to soil bacteria appeared, but peaks of the structural groups of smectitealso modified by the interaction with soil bacteria. It shows that some products of metabolism of soil bacteria have intercalated into thesmectite interlayer, and resulted in increasing ofbasalspacing. Comparing the effects of two soil bacteria, the gram-positive bacterium presented stronger effects.

赵玉连1，代群威1,2**，董发勤2，蒋沁芮1，唐俊1，陈武3. 两株土壤菌与蒙脱石相互作用的实验研究*[J]. 南京大学学报(自然科学版), 2013, 49(6): 767–.

Zhao Yu-Lian1, Dai Qun-Wei1,2, Dong Fa-Qin2, Jiang Qin-Rui1,Tang Jun1, ChenWu3. Experimental study oninteraction between two soil bacteria andsmectite[J]. Journal of Nanjing University(Natural Sciences), 2013, 49(6): 767–.

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