南京大学学报(自然科学版) ›› 2013, Vol. 49 ›› Issue (6): 718–.

• • 上一篇    下一篇

硫酸盐还原菌EPS)去除重金属离子Cd2+过程中的交互作用研究

彭书传,虞艳云,万正强,李明明,陈天虎,岳正波**   

  • 出版日期:2014-01-14 发布日期:2014-01-14
  • 作者简介:(合肥工业大学资源与环境工程学院,合肥230009)
  • 基金资助:
    国家自然科学基金(41130206,41102214),国家重点基础研究发展规划(973)预研项目(2011CB411904)

Research on the interaction of sulfate reducing bacteria EPS and the heavy metal of Cd2+

Peng Shu-Chuan, Yu Yan-Yun, Wan Zheng-Qiang, Li Ming-Ming , Chen Tian-hu, Wang Jin, Yue Zheng-Bo   

  • Online:2014-01-14 Published:2014-01-14
  • About author: (School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, 230009, China)

摘要: 本文以硫酸盐还原菌(Sulfate reducing bacteria, SRB)为试验菌株,探讨了重金属Cd2+对SRB胞外聚合物(Extracellular polymeric substances, EPS)组分和含量在,分别测定总产气量、Cd2+浓度以及EPS中蛋白质、多糖、核酸含量。实验结果表明试验菌株对Cd2+的最大耐受浓度为28.0 mg ? L-1;反应结束后Cd2+的去除率可以达到99%,而其中EPS的贡献率最大可以达到10%;蛋白质是SRB菌EPS的最主要组分;固态样品的SEM-EDS表征结果显示Cd2+与H2S生成CdS沉淀从而达到了固定Cd2+的作用。

Abstract: In this paper, the sulfate-reducing bacteria (SRB) was used as the test strains to explore the impacts of Cd2+ on the component and content of extracellular polymeric substances (EPS) and the role of EPS in the removal of Cd2+. Total gas production and Cd2+ concentrations were determined during the different reaction stages of the test as well as the content of proteins, polysaccharides and nucleic acids in EPS. The experimental results showed that the maximum tolerated concentration of Cd2+ to the test strain was 28.0 mg ? L-1. The removal efficiency of Cd2+ can reach 99% at the end of the reaction, in which the contribution of EPS was 10%. Protein was the most important component of the EPS produced by SRB. Furthermore, SEM-EDS characterization of the solid samples showed that Cd2+ was fixed by the generation of CdS precipitation with H2S produced.

[1] Buchanan R E , Gibbons N E . Bergey’s manual of determinati-ve bacteriology ( 8th ed ) [M] . Beijing : Science Press , 1984 : 663~679 (布坎南 R E , 吉本斯 N E . 伯杰氏细菌鉴定手册 ( 8 ) . 北京 : 科学出版社 . 1984 : 663~679 ) .

[2] Jin X,Wang J,Chen T H,et al.The synergistic influence of iron oxide on the dissolution of sulfate mineral.Acta Mineralogica Sinica,2010,30(3):343~348. ( , ,陈天虎,.铁氧化物对硫酸盐还原菌分解硫酸盐矿物的协同作用.矿物学报,2010,30(3):343~348).

[3] Wang X F,Xiang W Y,Yang J.Experiment on treating the heavy Cr ion wastewater by sulfate-reducing bacteria.Journal of Water Resources and Water Engineering,2012,23(2): 169~171.(王晓菲,向文英, .硫酸盐还原菌处理重金属铬离子废水的实验研究.水资源与水工程学报,2012,23(2):169~171).

[4] Kieu H T Q,Müller E,Horn H.Heavy metal removal in anaerobic semi-continuous stirred tank reactors by a consortium of sulfate-reducing bacteria.Water Research, 2011,45(13):3863~3870.

[5] Frolund B,Palmgren R,Keiding K,et al .Extraction of extracellular polymers from activated sludge using a cation exchange resin.Water Research,1996,30(8):1749~1758.

[6] Omoike A,Chorover J.Spectroscopic study of extracellular polymeric substances from Bacillus subtilis : aqueous chemistry and adsorption effects.Biomacromolecules,2004,5 (4):1219~1230.

[7] Flemming H C,Neu T R,Wozniak D J.The EPS matrix: thehouse of biofilm cells”.Journal of Bacteriology,2007, 189(22):7945~7947.

[8] Pal A,Paul A K.Microbial extracellular polymeric substances: central elements in heavy metal bioremediation.Indian Journal of Microbiology ,2008 ,48(1):49~64.

[9] Ras M,Lefebvre D,Derlon N,et al.Extracellular polymeric substances diversity of biofilms grown under contrasted environmental conditions. Water Research,2011,45(4): 1529~1538.

[10] Shen R,Sheng G P,Yu H Q.Determination of main components in the extracellular polymeric substances extracted from activated sludge using a spectral probing method. Biointerfaces,2012,94:151~156.

[11] Wingender J,Neu T R,Flemming H C.Microbial extracellular polymeric substances:characterization,structures and function. Springer-Verlag: Berlin Heidelberg,1999,30~31.

[12] Christensen B E, Kjosbakken J, Smidsrod O. Partial chemical and physical characterization of two extracellular polysaccharides produced by marine periphytic Pseudomonas sp. Strain NCMB 2021.Applied and Environment Microbiology,1985,50(4):837~845.

[13] Sheng G P,Yu H Q,Yu Z.Extraction of the extracellular polymeric substances from a photosynthetic bacterium Rhodopseudomonas acidophila. Applied Microbiology and Biotechnology,2005,67:125~130.

[14] Kang C L,Su C Y,Guo P,et al.Studies on Pb2+ and Cd2+ adsorption by extracellular protein of natural biofilm. Chemical Journal of Chinese Universities,2006,27(7): 1245~1246.(康春莉,苏春彦, ,.自然水体生物膜胞外蛋白质吸附铅和镉的研究.高等学校化学学报, 2006,27(7):1245~1246).

[15] Zheng Y,Fang X L,Ye Z L,et al.Biosorption of Cu(II)on extracellular polymers from Bacillus sp. F19.Journal of Environmental Sciences,2008,20(11):1288~1293.

[16] Liu Y,Lam M C,Fang H H P.Adsorption of heavy metals by EPS of activated sludge.Water Science and Technology, 2001,43:59~66.

[17] Wei X,Fang L,Cai P, et a1.Influence of extracellular polymeric substances(EPS)on Cd adsorption by bacteria. Environmental Pollution,2011,159(5):1369~1374.

[18] Balows A,Truper H G,Dworkin M,et al.The Prokaryotes. Vol IV, 2nd ed.New York Springer-Verlag,1992,3(6): 352~378.

[19] Liu Q W,Zhang Z J,Lu J,et al.Study on the domestication and desulphurization of the sulfate-reducing bacteria. Proceedings of the CSEE,2009,29(17):51~55.(刘启旺,张知见, ,.硫酸盐还原菌的驯化及脱硫性能研究.中国电机工程学报,2009,29(17):51~55).

[20] Gaudy A F.Colorimetric determination of protein and carbohydrate. Industry Water Wastes,1962,7:17~22.

[21] Raunkjær K,Hvitved-Jacobsen T, Nielsen P H.Measurement of pools of protein,carbohydrate and lipid in domestic wastewater.Water Research,1994,28(2):251~261.

[22] Zhong W Z,Zhang Z Z,Luo Y J,et al .Biogas productivity by co-digesting Taihu blue algae with corn straw as an external carbon source. Bioresource Technology,2012,114:281~286.

[23] Sheng G P,Yu H Q,Yue Z B.Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presences of toxic substances. Applied Microbiology and Biotechnology,2005,69:216~222.

[24] Cao X S,Long T R,Meng X Z,et al.Effects of Mn2+,Mo6+ and Zn2+ on the components change of extracellular polymeric substances in activated sludge.Environmental Science, 2004,25(4):70~73.(曹相生,龙腾锐,孟雪征,.Mn2+Mo6+Zn2+对活性污泥内胞外聚合物组分的影响.环境科学,2004,25(4):70~73).

[25] Zhang D Y,Zhao Y S,Pan X L.The role of EPS in removing cadmium in sewage by algae bacteria biofilm. Research of Environmental Sciences,2004,17(5):52 ~55.(张道勇,赵勇胜,潘响亮.胞外聚合物(EPS)在藻菌生物膜去除污水中Cd的作用.环境科学研究,2004,17(5):52 ~55).

[26] Yin Y R,Hu Y Y,Xiong F.Sorption of Cu(II)and Cd(II)by extracellular polymeric substances(EPS)from Aspergillus fumigatus.International Biodeterioration & Biodegradation, 2011,65(7):1012~1018.

[27] Joshi N,Ngwenya B T,French C E.Enhanced resistance to nanoparticle toxicity is conferred by overproduction of extracellular polymeric substances.Journal of Hazardous Materials,2012,241-242(30):363~370.

[28] Su Y,Wang J,Peng S C,et al.Rice straw and sewage sludge as carbon sources for sulfate-reducing bacteria treating acid mine drainage.Environmental Science,2010,31(8): 1858~1863.( , ,彭书传,.以稻草和污泥为碳源硫酸盐还原菌处理酸性矿山排水.环境科学,2010,31(8):1858~1863).

[29] Hsieh K M, Murgel G A,Lion L W,et al.Interactions of microbial biofilms with toxic trace metals:2.Prediction and verification of an integrated computer model of lead(II) distribution in the presence of microbial activity. Biotechnology and Bioengineering,1994,44(2):232~239 .

[30] Suh H H,Kwon G S,Lee C H,et al.Characterization of bioflocculant produced by Bacillus sp. DP-152.J Ferment Bioeng,1997,84:108~112.

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