|本期目录/Table of Contents|

[1]赵莹莹,王永峰,孙棐斐,等. 四溴双酚A(TBBPA)好氧降解菌Pseudomonas sp.JDT 的筛选及其对TBBPA的降解转化[J].南京大学学报(自然科学),2017,53(2):218.[doi:10.13232/j.cnki.jnju.2017.02.002]
 Zhao Yingying,Wang Yongfeng,Sun Feifei,et al. Aerobic degradation of Tetrabranobisphenol A(TBBPA) by Pseudomonas sp.JDT isolated from an activated sludge[J].Journal of Nanjing University(Natural Sciences),2017,53(2):218.[doi:10.13232/j.cnki.jnju.2017.02.002]

 四溴双酚A(TBBPA)好氧降解菌Pseudomonas sp.JDT 的筛选及其对TBBPA的降解转化()




 Aerobic degradation of Tetrabranobisphenol A(TBBPA) by Pseudomonas sp.JDT isolated from an activated sludge
 赵莹莹王永峰孙棐斐郭红岩季 荣*
 Zhao YingyingWang YongfengSun FeifeiGuo HongyanJi Rong*
 State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment, Nanjing University,Nanjing,210023,China
 TBBPAmicrobial degradationPseudomonas14C tracer
 四溴双酚A(TBBPA)作为一种被广泛使用的溴代阻燃剂,在环境中广泛存在.从活性污泥中筛选出一株TBBPA的好氧降解菌,经16S rDNA测序鉴定为假单胞菌属,命名为Pseudomonas sp.JDT.该菌在40天内对TBBPA的脱溴率为12.9%.外加碳源能够促进该菌对TBBPA的矿化,且低浓度酵母提取物的促进作用高于葡萄糖和高浓度酵母提取物.利用14C示踪技术,在持续加有外来碳源的纯菌培养体系中测定JDT对TBBPA的降解,发现18.5天后TBBPA的矿化率为2.7%,并检测到两种极性代谢产物,分别占有机可提取部分总放射性量的11.2%和47.2%.Pseudomonas sp.JDT可以作为研究环境中TBBPA微生物降解途径的实验材料.
 A TBBPA­degrading aerobic bacterial strain was isolated from an activated sludge and was identified as a member of the genus Pseudomonas according to 16S rDNA gene analysis.The strain was named as Pseudomonas sp.JDT.After 40­day incubation,12.9% of TBBPA was debrominated by Pseudomonas sp.JDT.Extra carbon addition enhanced TBBPA mineralization by JDT.The inductive effects from yeast extract at a lower concentration was higher than that at a higher concentration.In the presence of extra carbon sources,2.7% of initial TBBPA was mineralized after 18.5 days incubation.Two metabolites with a higher polarity than TBBPA were detected at the end of incubation,accounting for 11.2% and 47.2% of extractable radioactivity,respectively.The results suggest that Pseudomonas sp.JDT may be used for study on the biodegradation pathway of TBBPA in the environment.


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更新日期/Last Update: 2017-03-25