|本期目录/Table of Contents|

[1]葛家春·,宋伟董张及,许志强,等. “全鱼”转生长激素基因黄颗鱼首建者的建立*[J].南京大学学报(自然科学),2013,49(1):123-131.[doi:10.13232/j.cnki.jnju.2013.01.015]
 Ge Jia-Chun,Song Wei,Dong ZhangJi,et al.Generation of "all fish" growth hormone gene transgenic yellow catfish founders[J].Journal of Nanjing University(Natural Sciences),2013,49(1):123-131.[doi:10.13232/j.cnki.jnju.2013.01.015]
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 “全鱼”转生长激素基因黄颗鱼首建者的建立*()
     

《南京大学学报(自然科学)》[ISSN:0469-5097/CN:32-1169/N]

卷:
49
期数:
2013年第1期
页码:
123-131
栏目:
出版日期:
2013-01-30

文章信息/Info

Title:
Generation of "all fish" growth hormone gene transgenic yellow catfish founders
作者:
 葛家春1·2宋伟3董张及3许志强12鲍洁3周国勤4潘建林2杨家新1赵庆顺3**
 (1.南京师范大学生命科学学院,南京,210046;2.江苏省淡水水产研究所,南京,210017;
3.南京大学模式动物研究所,南京,210061;.南京市水产科学研究所,南京,210036)
Author(s):
 Ge Jia-Chun1’2Song Wei3Dong Zhang一Ji3X u Zhi一Qiang1.2Bao Jie3 Zhou Guo一Qin4Pan Jian一Lin2Yang Jia一xin1Zhao Qing-Shun3
 (1 .College of Life Scicnces,Nanjing Normal University,Nanjing,210046,China;
2. Freshwater Fisheries Research institute of Jiangsu Province,Nanjing,210017,China;
3. Model Animal Research Center,Nanjing University,Nanjing,210061,China;
4. Nanjing Aquatic Sciences Institute,Nanjing,210036,China)
关键词:
 黄颡鱼生长激素全角转基因构件转基因
Keywords:
 Pelteobagrus fulvidracogrowth hormone"all-fish" transgenic constructtransgene
分类号:
-
DOI:
10.13232/j.cnki.jnju.2013.01.015
文献标志码:
-
摘要:
黄颡鱼(Pelteobagrus fulvidraco)是一种重要的淡水名优经济角鱼类,然而较小的体型和较慢
的生长速率极大地降低了它的经济价值.为获得大规格且快速生长的黄颖角,开展了转生长激素基因黄
颖角的研究.运用RT-PCR和RACE-PCR技术,克降了黄颖角生长激素基因的603 bp(碱基对)的编码
序列和485 bp,的3’-UTR(非翻译区)序列.通过重叠PCR和限制性内切酶酶切连接,组建了一个长为
2105 bp的“全鱼”转生长激素基因构件,该构件含1017 bp的黄颡鱼β-肌动蛋白近端启动子、603 bp的
生长激素基因编码序列和485 bp,的3’-UTR序列.采用显微注射将转生长激素基因构件导入黄颖角受
精卵,从301尾由注射肛胎发育而来的黄颖角中共筛选获得40尾转生长激素基因黄颖角首建者.成功
地建立了黄颡鱼基因组改造的技术平台,为黄颡鱼的基因工程育种奠定了基础.
Abstract:
 Yellow catfish(Pelteobagrus fulivdraco Richardson)is one of the most important freshwater farmed spe-
cies in China. However, its small size and slow growth rate limit its economic value. Urowth hormone is a protein se-
creted by pituitary, It promotes animal growth. Overexpression of growth hormone in fishes results in the growth
hormone gene transgenic fishes with enlarged body size and accelerated growth, In order to increase its body size and
growth rate,we performed transgenic research on yellow catfish. Employing RT-PCR and RACE(rapid amplification
of cDNA ends)-PCR,we cloned 603 bp(base pairs)complete coding sequence and X85 by 3,-UTR(untranslated re-
gion)of yellow catfish growth hormone gene,respectively. Performing overlapping PLR and molecular cloning with
restriction endonuclcases,we constructed an "all fish" growth hormone gene transgenic construct with 2105 by in
length. The construct comprises 1017 by of yellow catfish份actin proximal promoter, 603 by complete coding se
qucnce and 485 by 3’-UTR of yellow catfish growth hormone gene, respectively. Screening 301 individuals developed
from the yellow catfish fertilized eggs microinjected with the growth hormone gene transgenic construct,we obtained
40 transgenic founders. Our results showed that we have established a yellow catfish transgenic platform that lays
the foundation for our further molecular breeding to create new strains of yellow catfish with large body size and rap-
id growth.

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备注/Memo

备注/Memo:
 江苏省自然科学基金(BK2008484),江苏省科技支撑计划项日(BE2010376)
更新日期/Last Update: 2015-09-17